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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/8680
Title: 
Adsorption and detection of DNA dendrimers at carbon electrodes
Author(s): 
Institution: 
  • New Mexico State Univ
  • Polyprobe Inc
  • Univ Nacl Cordoba
  • Universidade Estadual Paulista (UNESP)
  • Univ Valencia
ISSN: 
1040-0397
Abstract: 
Dendritic nucleic acids are highly branched and ordered molecular structures, possessing numerous single-stranded oligonucleotide arms, which hold great promise for enhancing the sensitivity of DNA biosensors. This article evaluates the interfacial behavior and redox activity of nucleic acid dendrimers at carbon paste electrodes, in comparison to DNA. Factors influencing the adsorption behavior, including the adsorption potential and time, solution conditions, or dendrimer concentration, are explored. The strong adsorption at the anodically pretreated carbon surface is exploited for an effective preconcentration step prior to the chronopotentiometric measurement of the surface species. Coupled with the numerous guanine oxidation sites, such stripping protocol offers remarkably low detection limits (e.g., 3 pM or 2.4 femtomole of the I-layer dendrimer following a 15 min accumulation). The new observations bear important implications upon future biosensing applications of nucleic dendrimers.
Issue Date: 
1-Jul-1998
Citation: 
Electroanalysis. Berlin: Wiley-v C H Verlag Gmbh, v. 10, n. 8, p. 553-556, 1998.
Time Duration: 
553-556
Publisher: 
Wiley-Blackwell
Keywords: 
  • dendrimers
  • nucleic acids
  • adsorptive potentiometry
  • DNA
  • biosensors
  • carbon paste
Source: 
http://dx.doi.org/10.1002/(SICI)1521-4109(199807)10:8<553
URI: 
Access Rights: 
Acesso restrito
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/8680
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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