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Meeting in the middle before parting: metaphase: merotelic [Mitosis movie series; 4]
  • Presents metaphase PtK1 cell microinjected with X-rhodamine-labeled tubulin and Alexa 488-labeled CENP-F antibodies, to fluorescently label kinetochore fibers (red) and kinetochores/spindle poles (green), respectively. The movie shows that sister kinetochore pairs oscillate between the two spindle poles as their respective kinetochore fibers lengthen and shorten. The top kinetochore of the first sister pair on the right exhibits abnormal attachment to spindle microtubules, as it is bound to microtubules coming from both spindle poles, rather than just one (merotelic kinetochore orientation). As a consequence of this mis-attachment, the merotelically oriented kinetochore remains relatively stationary while its normally oriented sister oscillates between poleward and anti-poleward movements
  • Componente Curricular::Educação Superior::Ciências Biológicas::Biologia Geral
Issue Date: 
  • 11-May-2009
  • 11-May-2009
  • Aug-2006
  • 11-May-2009
  • 2-Oct-2008
The American Society for Cell Biology, ASBC Image & Video Library
  • Mitosis
  • Metaphase
  • Kinetochore
  • Educação Superior::Ciências Biológicas::Biologia Geral
To show the sister kinetochore pairs oscillate between the two spindle poles
Duration: 9 s. Original video created on October 22, 2003. Original resource provided by Daniela Cimini (Virginia Polytechnic Institute, Blacksburg, VA). Work conducted at University of North Carolina, Chapel Hill, NC. This video was categorizes by the site as: [Mitosis Movie Series: 4]. To visualize this source its necessary the instalation of Quick time. Available at: <>
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Appears in Collections:MEC - Objetos Educacionais (BIOE) - OE

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