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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/unesp/361015
Title: 
PIP3 produced in response to stimulation by a growth factor
Author(s): 
Field, Seth J.
Language: 
nau
Description: 
  • Movie of an NIH 3T3 mouse fibroblast that has been starved for growth factors and then stimulated by platelet-derived growth factor (PDGF). The cell is expressing a reporter for PIP3 (phosphatidylinositol(3,4,5)trisphosphate), composed of a PIP3 binding domain (the pleckstrin homology domain of Bruton's Tyrosine Kinase) fused to enhanced green fluorescent protein (EGFP). In the starved cell the reporter is partially nuclear (likely due to a weak nuclear localization sequence within EGFP). Beginning at the third frame, in response to 50ng/ml PDGF, the reporter rapidly reveals the production of the lipid second messenger PIP3 at the plasma membrane
  • Componente Curricular::Educação Superior::Ciências Biológicas::Morfologia
Issue Date: 
  • Aug-2007
  • 22-May-2009
  • 22-May-2009
  • 22-May-2009
  • 2-Oct-2008
Publisher: 
The American Society for Cell Biology, ASCB Image & Video Library
Keywords: 
  • Fibroblast
  • Cell
  • Educação Superior::Ciências Biológicas::Morfologia::Citologia e Biologia Celular
Notes: 
To show a movie of an NIH 3T3 mouse fibroblast that has been starved for growth factors and then stimulated by platelet-derived growth factor (PDGF)
Credits: 
Duration: 7 s. Original movie created on January 16, 2001. Work conducted at University of California, San Diego, La Jolla, CA. Software required: Apple Quicktime 7 player. Available at: <http://www.apple.com/quicktime/download/win.html>
Source: 
http://objetoseducacionais2.mec.gov.br/handle/mec/10322
URI: 
http://acervodigital.unesp.br/handle/unesp/361015
Rights: 
You may use the Images only for research and educational purposes in an educational institution. Images may be stored on a network server, hard drive or removable media, but only while this Agreement remains in effect. All of the Images you use must be accompanied by a notice stating, "© [Date and Author's or Owner's Name]. All rights reserved. Reprinted under license from The American Society for Cell Biology." The notice shall appear with the Images or on a credits page. "Educational purposes" means (i) non-commercial instruction or curriculum based teaching by educators to students at nonprofit educational institutions, (ii) planned non-commercial study, investigation or research directed toward making a contribution to a field of knowledge, or (iii) presentation of research findings at non-commercial peer conferences, workshops or seminars, but in any event there must be express written permission from ASCB before an Image can be published. "Educational institutions" include K-12 schools, colleges and universities. Libraries, museums, hospitals, research institutes and other nonprofit organizations are considered educational institutions when they engage in nonprofit instructional, research or scholarly activities for educational purposes
Type: 
vídeo
Appears in Collections:MEC - Objetos Educacionais (BIOE) - OE

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