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PIP3 produced in response to stimulation by a growth factor
Field, Seth J.
  • Movie of an NIH 3T3 mouse fibroblast that has been starved for growth factors and then stimulated by platelet-derived growth factor (PDGF). The cell is expressing a reporter for PIP3 (phosphatidylinositol(3,4,5)trisphosphate), composed of a PIP3 binding domain (the pleckstrin homology domain of Bruton's Tyrosine Kinase) fused to enhanced green fluorescent protein (EGFP). In the starved cell the reporter is partially nuclear (likely due to a weak nuclear localization sequence within EGFP). Beginning at the third frame, in response to 50ng/ml PDGF, the reporter rapidly reveals the production of the lipid second messenger PIP3 at the plasma membrane
  • Componente Curricular::Educação Superior::Ciências Biológicas::Morfologia
Issue Date: 
  • Aug-2007
  • 22-May-2009
  • 22-May-2009
  • 22-May-2009
  • 2-Oct-2008
The American Society for Cell Biology, ASCB Image & Video Library
  • Fibroblast
  • Cell
  • Educação Superior::Ciências Biológicas::Morfologia::Citologia e Biologia Celular
To show a movie of an NIH 3T3 mouse fibroblast that has been starved for growth factors and then stimulated by platelet-derived growth factor (PDGF)
Duration: 7 s. Original movie created on January 16, 2001. Work conducted at University of California, San Diego, La Jolla, CA. Software required: Apple Quicktime 7 player. Available at: <>
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Appears in Collections:MEC - Objetos Educacionais (BIOE) - OE

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