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- Dancing spaghetti: the effect of BFA on the TGN [4b. Honorable mention]
- Mardones, Gonzalo A.
- Brefeldin A (BFA) is a fungal metabolite that reversibly blocks protein transport from the endoplasmic reticulum (ER) to the Golgi apparatus. This effect is accompanied by a distinctive morphological change, the reversible collapse of the Golgi apparatus. The disassembly of the Golgi apparatus is accompanied by its dramatic tubulation, forming a network extending throughout the cytoplasm. While the content of the Golgi apparatus is redistributed to the ER, the content of the trans-Golgi network (TGN) collapses in the vicinity of the MTOC. This video shows the effect of BFA in an NRK cell stably expressing GFP-GMx33a, a protein associated to the TGN. To visualize tubulation with high temporal and spatial resolution, capture of images started after 10 min of BFA addition
- Componente Curricular::Educação Superior::Ciências Biológicas::Biologia Geral
- The American Society for Cell Biology, ASCB Image & Video Library
- Educação Superior::Ciências Biológicas::Biologia Geral
- To show the effect of BFA in an NRK cell stably expressing GFP-GMx33a, a protein associated to the TGN
- Duration: 34 s. This video was categorized by the site as: [Celldance 2007: 4b. honorable mention]. To visualize this source its necessary the instalation of Quick time. Available at: <http://www.apple.com/pt/quicktime/>
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