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|dc.contributor.author||Mardones, Gonzalo A.||-|
|dc.description||Brefeldin A (BFA) is a fungal metabolite that reversibly blocks protein transport from the endoplasmic reticulum (ER) to the Golgi apparatus. This effect is accompanied by a distinctive morphological change, the reversible collapse of the Golgi apparatus. The disassembly of the Golgi apparatus is accompanied by its dramatic tubulation, forming a network extending throughout the cytoplasm. While the content of the Golgi apparatus is redistributed to the ER, the content of the trans-Golgi network (TGN) collapses in the vicinity of the MTOC. This video shows the effect of BFA in an NRK cell stably expressing GFP-GMx33a, a protein associated to the TGN. To visualize tubulation with high temporal and spatial resolution, capture of images started after 10 min of BFA addition||-|
|dc.description||Componente Curricular::Educação Superior::Ciências Biológicas::Biologia Geral||-|
|dc.publisher||The American Society for Cell Biology, ASCB Image & Video Library||-|
|dc.rights||You may use the Images only for research and educational purposes in an educational institution. Images may be stored on a network server, hard drive or removable media, but only while this Agreement remains in effect. All of the Images you use must be accompanied by a notice stating, "© [Date and Author's or Owner's Name]. All rights reserved. Reprinted under license from The American Society for Cell Biology." The notice shall appear with the Images or on a credits page. "Educational purposes" means (i) non-commercial instruction or curriculum based teaching by educators to students at nonprofit educational institutions, (ii) planned non-commercial study, investigation or research directed toward making a contribution to a field of knowledge, or (iii) presentation of research findings at non-commercial peer conferences, workshops or seminars, but in any event there must be express written permission from ASCB before an Image can be published. "Educational institutions" include K-12 schools, colleges and universities. Libraries, museums, hospitals, research institutes and other nonprofit organizations are considered educational institutions when they engage in nonprofit instructional, research or scholarly activities for educational purposes||-|
|dc.subject||Educação Superior::Ciências Biológicas::Biologia Geral||-|
|dc.title||Dancing spaghetti: the effect of BFA on the TGN [4b. Honorable mention]||-|
|dc.description2||To show the effect of BFA in an NRK cell stably expressing GFP-GMx33a, a protein associated to the TGN||-|
|dc.description3||Duration: 34 s. This video was categorized by the site as: [Celldance 2007: 4b. honorable mention]. To visualize this source its necessary the instalation of Quick time. Available at: <http://www.apple.com/pt/quicktime/>||-|
|Appears in Collections:||MEC - Objetos Educacionais (BIOE) - OE|
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