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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/unesp/361051
Title: 
Metaphase kinetochore dynamics [1c. First place]
Author(s): 
Language: 
nau
Description: 
  • In this HeLa cell, which is expressing both a kinetochore marker (EGFP-CENP-B) and a centrosome marker (Venus-centrin), one can visualize the dynamic nature of kinetochore movements during metaphase. At this stage in mitosis, sister kinetochore pairs, seen as paired dots oriented parallel to the x-axis, are attached to opposite spindle poles, marked by the two pairs of venus centrin dots near the left and right edges of the movie (usually oriented perpendicular to the x-axis in this movie). Kinetochores in metaphase HeLa cells typically undergo oscillatory movements around the equator of the spindle with average oscillation amplitudes of 1.2 microns. The sister kinetochores display primarily coordinated movements but also move relative to each other, which can be seen as increases and decreases in the distance between the sisters. These movements are highly coordinated with the dynamics of microtubules attached to the kinetochores. Note that the centrin dots are approximately 12 microns apart at the start of the movie
  • Componente Curricular::Educação Superior::Ciências Biológicas::Biologia Geral
Issue Date: 
  • 12-May-2009
  • 12-May-2009
  • 20-Aug-2007
  • 12-May-2009
  • 6-Oct-2008
Publisher: 
The American Society for Cell Biology, ASCB Image & Video Library
Keywords: 
  • Cell biology
  • Microtubule
  • Mitosis
  • Mitotic spindle
  • Kinetochore
  • Educação Superior::Ciências Biológicas::Biologia Geral
Notes: 
To show metaphase kinetochore dynamics
Credits: 
Duration: 9 s. Contributors: Mike Wagenbach (University of Washington, Seattle, WA) and Benjamin Major (University of Washington, Seattle, WA). This video was categorized by the site as: [Celldance 2007: 1c. First place]. Software required: Apple Quicktime 7 player. Available at: <http://www.apple.com/quicktime/download/win.html>
Source: 
http://objetoseducacionais2.mec.gov.br/handle/mec/10254
URI: 
http://acervodigital.unesp.br/handle/unesp/361051
Rights: 
You may use the Images only for research and educational purposes in an educational institution. Images may be stored on a network server, hard drive or removable media, but only while this Agreement remains in effect. All of the Images you use must be accompanied by a notice stating, "© [Date and Author's or Owner's Name]. All rights reserved. Reprinted under license from The American Society for Cell Biology." The notice shall appear with the Images or on a credits page. "Educational purposes" means (i) non-commercial instruction or curriculum based teaching by educators to students at nonprofit educational institutions, (ii) planned non-commercial study, investigation or research directed toward making a contribution to a field of knowledge, or (iii) presentation of research findings at non-commercial peer conferences, workshops or seminars, but in any event there must be express written permission from ASCB before an Image can be published. "Educational institutions" include K-12 schools, colleges and universities. Libraries, museums, hospitals, research institutes and other nonprofit organizations are considered educational institutions when they engage in nonprofit instructional, research or scholarly activities for educational purposes.
Type: 
vídeo
Appears in Collections:MEC - Objetos Educacionais (BIOE) - OE

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