Efeitos da suplementação energética e lipídica no perfil metabólico, desenvolvimento folicular e produção in vitro de embrióes em novilhas da raça Nelore (Bos taurus indicus)

Abstract

O objetivo deste estudo foi verificar os efeitos da suplementação energética e com diferentes fontes de gordura na produção de embriões FIV e no perfil metabólico em novilhas nelore. Foram realizados dois experimentos, no primeiro, vinte novilhas Nelore (Bos taurus indicus) com idade média de 30 meses, com peso médio de 417,0 ± 42,4 kg foram distribuidas aleatoriamente em 3 tratamentos: T1 (n= 7), animais receberam 100% das exigências de manutença de energia, calculados conforme NRC (1997), T2 onde os animais receberam 170% das exigências de manutenção de energia (1,7 X M), e T3, onde os animais receberam 170 % das exigências de manutenção de energia com adição de 200 g de gordura protegida-AGCL (Megalac®). No segundo experimento 12 novilhas Nelore (Bos taurus indicus) com idade variando de 14 a 18 meses, com peso médio inicial de 309,6 kg, foram distribuidas em 4 tratamentos, de acordo com peso e população folicular avaliada por ultrasonografia: T1, animais receberam 100% dos requerimentos de mantença de energia, calculados conforme NRC (1997), T2- animais receberam 170% das exigências de mantença de energia, T3- idem ao T2, com adição de 4% de óleo de soja na MS, e T4- idem ao T2, com adição de 200 g de AGCL (Megalac ®). O período de adaptação foi quinze dias, onde os animais receberam feno de capim Brachiaria e ração concentrada...

Two experiments were carried to evaluate the effect of the energy and fat in the metabolic profile, follicular dynamic, oocyte and in vitro embryo production in Nelore heifers (Bos taurus indicus). In the first, twenty Nelore heifers with 30 months old, and average weight 417,0 ± 42,4 were allocated in 3 treatments, in accordance with weight and follicular population evaluated by ultrasound: T1, animals received 100% of energy maintenance, calculated as NRC (1997), T2- animals received 170% of energy maintenance; T3: animals received 170% of energy maintenance with addition of 200 g Megalac ®. In the second experiment, 12 Nelore heifers with 14-18 months and 309,6 kg average weight, were allocated in 3 treatments, in tree periods: T1, animals received 100% of energy maintenance, calculated as NRC (1997), T2- animals received 170% of energy maintenance; T3: animals received 170% of energy maintenance with addition of 4% soybean oil in Dry Matter and T4: animals received 170% of energy maintenance with addition of 200 g Megalac ®. After adaptation period, the treatment initiated, animals had synchronized ovulation, blood samples were collected, and follicular aspiration was carried to evaluate the oocytes and in vitro embryo production. Follicular development was monitored daily by ultrasonography until day post heat. The consumption of MS and the weight gain were lower in group T1 (P< 0.05), in relation to the others treatments. The FSH, LH, and albumin concentrations were not different (P > 0.05) among treatments. The cholesterol concentrations were higher in the T3 and T4 groups (P< 0.05) only in the second experiment... (Complete abstract, click electronic access below)