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http://acervodigital.unesp.br/handle/11449/108
- Title:
- On the temperature stability of extracellular hemoglobin of Glossoscolex paulistus, at different oxidation states: SAXS and DLS studies
- Universidade de São Paulo (USP)
- Universidade Estadual Paulista (UNESP)
- 0301-4622
- Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
- Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
- FAPESP: 10/09719-0
- Glossoscolex paulistus hemoglobin (HbGp) was studied by dynamic light scattering (DLS) and small angle X-ray scattering (SAXS). DLS melting curves were measured for met-HbGp at different concentrations. SAXS temperature studies were performed for oxy-, cyanomet- and met-HbGp forms, at several pH values. At pH 5.0 and 6.0, the scattering curves are identical from 20 to 60 degrees C, and R-g is 108 angstrom, independent of the oxidation form. At pH 7.0, protein denaturation and aggregation occurs above 55 degrees C and 60 degrees C, for oxy and met-HbGp, respectively. Cyanomet-HbGp, at pH 7.0, is stable up to 60 degrees C. At alkaline pH (8.0-9.0) and higher temperature, an irreversible dissociation process is observed, with a decrease of R-g, D-max and I(0). Analysis by p(r), obtained from GNOM, and OLIGOMER, was used to fit the SAXS experimental scattering curves by a combination of theoretical curves obtained for HbLt fragments from the crystal structure. Our results show clearly the increasing contribution of smaller molecular weight fragments, as a function of increasing pH and temperature, as well as, the order of thermal stabilities: cyanomet-> oxy- > met-HbGp. (C) 2012 Elsevier B.V. All rights reserved.
- 1-Apr-2012
- Biophysical Chemistry. Amsterdam: Elsevier B.V., v. 163, p. 44-55, 2012.
- 44-55
- Elsevier B.V.
- Extracellular hemoglobin
- Glossoscolex paulistus
- Oligomeric dissociation
- Thermal stability
- DLS
- SAXS
- http://dx.doi.org/10.1016/j.bpc.2012.02.004
- Acesso restrito
- outro
- http://repositorio.unesp.br/handle/11449/108
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