Penicillinase-based amperometric biosensor for penicillin G

Goncalves, Luis Moreira; Callera, Welder F. A.; Sotomayor, Maria Del Pilar Taboada; Bueno, Paulo Roberto

Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/111420

Title:

Penicillinase-based amperometric biosensor for penicillin G

Author(s):

Institution:

Univ Porto
Universidade Estadual Paulista (UNESP)

ISSN:

1388-2481

Sponsorship:

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Sao Paulo State University (UNESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Portuguese Fundacao para a Ciencia e a Tecnologia (FCT)

Sponsorship Process Number:

Portuguese Fundacao para a Ciencia e a Tecnologia (FCT)SFRH/BPD/76544/2011

Abstract:

A biosensor for penicillin G was created by immobilizing penicillinase to a gold electrode by means of a cysteine self-assembled monolayer. The biosensor amperometrically monitored the catalytic hydrolysis of penicillin in a very sensible manner. Furthermore, it was successfully used to measure the Michaelis-Menten enzymatic constant and a low limit of detection of 4.5 nM was obtained. (C) 2013 Elsevier B.V. All rights reserved.

Issue Date:

1-Jan-2014

Citation:

Electrochemistry Communications. New York: Elsevier Science Inc, v. 38, p. 131-133, 2014.

Time Duration:

131-133

Publisher:

Elsevier B.V.

Keywords:

Cysteine
Metallo-beta-lactamase
Self-assembled monolayer (SAM)
beta-Lactam antibiotic
Lineweaver-Burk plot

Source:

http://dx.doi.org/10.1016/j.elecom.2013.11.022

URI:

Access Rights:

Acesso restrito

Type:

outro

Source:

http://repositorio.unesp.br/handle/11449/111420

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