Temperature and storage period over spermatic parameters of jundia, Rhamdia quelen (Quoy & Gaimard, 1824)

Abstract

The aim of this work was to evaluate the spermatic parameters of jundia, Rhamdia quelen, semen stored for short periods under different temperatures. Fifteen males were used in a time factorial experimental design (13x3x3x3). The sperm was stored at: 15; 25 and 35 degrees C and activated in water at: 15; 25 and 35 degrees C each, respectively, within storage periods of: 0; 2; 4; 6; 8; 10; 12; 16; 20; 24; 32; 40 and 48h after collection. The treatments were performed in triplicates and in sequential protocols every 50h. The motility parameters were evaluated using Pearson's correlation analysis and the significant parameters (P<0.05) were summarized using principal component analysis forming two predicted groups. Group 01 composed the following parameters: curvilinear velocity, wobble and linearity; group 02 comprised average path velocity, straight line velocity, beating cross frequency and motility rate. Effects (P<0.05) of treatments were observed only in group 02, with interaction between time and temperature of exposure and between exposure time and water temperature. Superior results of spermatic parameters of group 02 were observed right after collection. However, the exposure and spermatic activation at 15 degrees C assured significant results 48h after collection when compared with 25 and 35 degrees C.