Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex

Spositto, F.l.e.; Campanerut, P.a.z.; Ghiraldi, L.d.; Leite, C.q.f.; Hirata, M.h.; Hirata, R.d.c.; Siqueira, V.l.d.; Cardoso, R. Fressatti

Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/114347

Title:

Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex

Author(s):

Institution:

Universidade Estadual de Maringá (UEM)
Universidade Estadual de Maringá (UEM)
Universidade Estadual Paulista (UNESP)
Universidade de São Paulo (USP)

ISSN:

1517-8382

Sponsorship:

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Abstract:

We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.

Issue Date:

1-Sep-2014

Citation:

Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 45, n. 3, p. 841-843, 2014.

Time Duration:

841-843

Publisher:

Sociedade Brasileira de Microbiologia

Keywords:

bovine tuberculosis
tuberculosis
PCR-multiplex
diagnosis

Source:

http://dx.doi.org/10.1590/S1517-83822014000300012

URI:

Access Rights:

Acesso aberto

Type:

outro

Source:

http://repositorio.unesp.br/handle/11449/114347

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