You are in the accessibility menu

Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/128469
Title: 
Improved embryonic cryosurvival observed after in vitro supplementation with conjugated linoleic acid is related to changes in the membrane lipid profile
Author(s): 
Institution: 
  • Universidade Estadual Paulista (UNESP)
  • Universidade Estadual de Campinas (UNICAMP)
  • Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
ISSN: 
0093-691X
Sponsorship: 
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Abstract: 
The aim of this study was to evaluate the effect of supplementing serum-containing media with a mixture of cis- and trans-9,11- and -10,12-conjugated isomers of linoleic acid (CIA) during different steps of the in vitro production (IVM, IVC, or IVM IVC) of bovine embryos on their embryonic development, cryotolerance, and lipid profile. To evaluate the impact of the CIA on membrane lipids, such as phosphatidylcholine (PC) and sphingomyelin (SM), the embryos'lipid profiles were obtained using matrix-assisted laser desorption ionization mass spectrometry. The cleavage rates (78.6%-84.8%) and blastocyst development (44.8%-51.2%) remained unaltered. The postthawing reexpansion rates were higher (P < 0.05) when the CIA was added to the IVM medium (82.6%) or to the IVM + IVC medium (83.8%) than the control (69.3%) or IVC medium (63.0%). Changes in the blastocysts'lipid profile occurred when supplementation was restricted to the IVM or IVC medium. However, the most prominent effects of the CIA on the embryonic PC and SM profiles were observed when the supplement was added to IVM + IVC media, which was an increase in the level of highly unsaturated PCs containing 36 or 38 carbons, which are likely to contain CIA residues. These results showed that the molecular mechanism resulting in the improved cryosurvival, observed with CIA supplementation during bovine embryonic in vitro production, was related to the composition of structural lipids of cellular membranes and is dependent on the treatment length. Monitoring the lipid profile of embryonic membranes may improve the CIA supplementation strategy and facilitate the development of new IVC systems to improve the cryopreservation of bovine embryos and those of other domestic species.
Issue Date: 
1-Jul-2015
Citation: 
Theriogenology. New York: Elsevier Science Inc, v. 84, n. 1, p. 127-136, 2015.
Time Duration: 
127-136
Publisher: 
Elsevier B.V.
Keywords: 
  • Bovine embryo
  • Conjugated linoleic acid
  • In vitro production
  • Mass spectrometry
  • Lipid profile
  • Cryosurvival
Source: 
http://www.sciencedirect.com/science/article/pii/S0093691X15001272
URI: 
Access Rights: 
Acesso restrito
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/128469
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.
 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.