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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/128702
Title: 
A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
Author(s): 
Institution: 
Universidade Estadual Paulista (UNESP)
ISSN: 
1517-8382
Sponsorship: 
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Abstract: 
A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.
Issue Date: 
1-May-2015
Citation: 
Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 46, n. 1, p. 113-116, 2015.
Time Duration: 
113-116
Publisher: 
Soc Brasileira Microbiologia
Keywords: 
  • Nitrile hydratase
  • Amidase
  • Nitrilase
  • Amidase inhibitor
  • High-throughput screening
Source: 
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100113&lng=en&nrm=iso&tlng=en
URI: 
Access Rights: 
Acesso aberto
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/128702
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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