Please use this identifier to cite or link to this item:
http://acervodigital.unesp.br/handle/11449/129404
- Title:
- Chronic nicotine activates stress/reward-related brain regions and facilitates the transition to compulsive alcohol drinking
- Universidade Estadual Paulista (UNESP)
- Scripps Res Inst
- NIDA
- 0270-6474
- National Institutes of Health (NIH)
- National Institute on Drug Abuse, Intramural Research Program, NIH
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
- National Institutes of Health (NIH): AA018914
- National Institutes of Health (NIH): AA020608
- National Institutes of Health (NIH): AA008459
- National Institutes of Health (NIH): AA006420
- National Institutes of Health (NIH): AA022977
- National Institutes of Health (NIH): DA023597
- CAPES: 0093/11-4
- Alcohol and nicotine are the two most co-abused drugs in the world. Previous studies have shown that nicotine can increase alcohol drinking in nondependent rats, yet it is unknown whether nicotine facilitates the transition to alcohol dependence. We tested the hypothesis that chronic nicotine will speed up the escalation of alcohol drinking in rats and that this effect will be accompanied by activation of sparsely distributed neurons (neuronal ensembles) throughout the brain that are specifically recruited by the combination of nicotine and alcohol. Rats were trained to respond for alcohol and made dependent using chronic, intermittent exposure to alcohol vapor, while receiving daily nicotine (0.8 mg/kg) injections. Identification of neuronal ensembles was performed after the last operant session, using immunohistochemistry. Nicotine produced an early escalation of alcohol drinking associated with compulsive alcohol drinking in dependent, but not in nondependent rats (air exposed), as measured by increased progressive-ratio responding and increased responding despite adverse consequences. The combination of nicotine and alcohol produced the recruitment of discrete and phenotype-specific neuronal ensembles (similar to 4-13% of total neuronal population) in the nucleus accumbens core, dorsomedial prefrontal cortex, central nucleus of the amygdala, bed nucleus of stria terminalis, and posterior ventral tegmental area. Blockade of nicotinic receptors using mecamylamine (1 mg/kg) prevented both the behavioral and neuronal effects of nicotine in dependent rats. These results demonstrate that nicotine and activation of nicotinic receptors are critical factors in the development of alcohol dependence through the dysregulation of a set of interconnected neuronal ensembles throughout the brain.
- 15-Apr-2015
- Journal Of Neuroscience. Washington: Soc Neuroscience, v. 35, n. 15, p. 6241-6253, 2015.
- 6241-6253
- Soc Neuroscience
- Addiction
- Alcohol
- Compulsivity
- Fos
- Neuronal ensembles
- Tobacco
- http://www.jneurosci.org/content/35/15/6241
- Acesso restrito
- outro
- http://repositorio.unesp.br/handle/11449/129404
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.