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Title: 
Extraction of natural red colorants from the fermented broth of Penicillium purpurogenum using aqueous two-phase polymer systems
Author(s): 
Institution: 
  • Universidade Estadual Paulista (UNESP)
  • Universidade de São Paulo (USP)
  • Universidade Federal do Amazonas (UFAM)
ISSN: 
1520-6033
Sponsorship: 
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Sponsorship Process Number: 
  • FAPESP: 2008/58280-0
  • FAPESP: 2013/08617-7
Abstract: 
Safety concerns related to the increasing and widespread application of synthetic coloring agents have increased the demand for natural colorants. Fungi have been employed in the production of novel and safer colorants. In order to obtain the colorants from fermented broth, suitable extraction systems must be developed. Aqueous two-phase polymer systems (ATPPS) offer a favorable chemical environment and provide a promising alternative for extracting and solubilizing these molecules. The aim of this study was to investigate the partitioning of red colorants from the fermented broth of Penicillium purpurogenum using an ATPPS composed of poly(ethylene glycol) (PEG) and sodium polyacrylate (NaPA). Red colorants partitioned preferentially to the top (PEG-rich phase). In systems composed of PEG 6,000 g/mol/NaPA 8,000 g/mol, optimum colorant partition coefficient (KC ) was obtained in the presence of NaCl 0.1 M (KC  = 10.30) while the PEG 10,000 g/mol/NaPA 8,000 g/mol system in the presence of Na2 SO4 0.5 M showed the highest KC (14.78). For both polymers, the mass balance (%MB) and yield in the PEG phase (%ηTOP ) were close to 100 and 79%, respectively. The protein selectivity in all conditions evaluated ranged from 2.0-3.0, which shows a suitable separation of the red colorants and proteins present in the fermented broth. The results suggest that the partitioning of the red colorants is dependent on both the PEG molecular size and salt type. Furthermore, the results obtained support the potential application of ATPPS as the first step of a purification process to recover colorants from fermented broth of microorganisms.
Issue Date: 
2015
Citation: 
Biotechnology Progress, v. 31, n. 5, p. 1295-1304, 2015.
Time Duration: 
1295-1304
Publisher: 
American Institute of Chemical Engineers
Keywords: 
  • Penicillium purpurogenum
  • Aqueous two-phase polymer systems
  • Extraction
  • Poly (ethylene glycol)
  • Red colorants
  • Sodium polyacrylate
Source: 
http://dx.doi.org/10.1002/btpr.2127
URI: 
Access Rights: 
Acesso restrito
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/131313
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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