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Utilize este identificador para citar ou criar um link para este item: http://acervodigital.unesp.br/handle/11449/14797
Título: 
A Modified Technique of Rat Tail Suspension for Longer Periods of Observation
Autor(es): 
Instituição: 
  • Universidade de São Paulo (USP)
  • Universidade Estadual Paulista (UNESP)
ISSN: 
0095-6562
Financiador: 
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Resumo: 
FALCAI MJ, LOUZADA MJQ, DE PAULA FJA, OKUBO R, VOLPON JB. A modified technique of rat tail suspension for longer periods of observation. Aviat Space Environ Med 2012; 83:1176-80. Background: Rat tail suspension is an accepted method to create experimental osteopenia. However, suspension periods longer than 3 wk may cause tail skin sloughing or rat slippage. The hypothesis was that a traction system with skeletal anchorage through one tail vertebra would prolong the suspension time without significant complications. Methods: There were 80 young adult female Wistar rats that were submitted to one of the following interventions: skeletal tail suspension (N = 20), skin tail suspension (N = 20), no intervention (N = 20), and a baseline control (N = 20). All animals were followed up either for 3 (N = 10) or 6 (N = 10) wk. Animals were assessed for clinical signs of stress and tolerance to suspension. The femur evaluation was in terms of mineral density content, mechanical resistance, and histomorphometry. Results/Discussion: All animals reached the 3-wk end point. However, for the 6-wk period, seven animals suspended by the skin traction method were discarded (70%) because of signs of stress and skin sloughing. In contrast, there was one loss in the skeletal suspension group (10%). All suspended animals developed similar osteopenia at 3 wk characterized by decreased bone mineral content, weakened bone resistance, and loss of femoral mass. At 6 wk, all suspended animals had similar osteopenic parameters, but they were not statistically different from those of the rats in the 3-wk groups. Therefore, suspension longer than 3 wk did not increase the bone deterioration in the femur.
Data de publicação: 
1-Dez-2012
Citação: 
Aviation Space and Environmental Medicine. Alexandria: Aerospace Medical Assoc, v. 83, n. 12, p. 1176-1180, 2012.
Duração: 
1176-1180
Publicador: 
Aerospace Medical Assoc
Palavras-chaves: 
  • skin suspension
  • skeletal suspension
  • osteopenia
  • hypoactivity
  • bone weakening
Fonte: 
http://dx.doi.org/10.3357/ASEM.3248.2012
Endereço permanente: 
Direitos de acesso: 
Acesso restrito
Tipo: 
outro
Fonte completa:
http://repositorio.unesp.br/handle/11449/14797
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