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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/15922
Title: 
Titanium surfaces with nanotopography modulate cytokine production in cultured human gingival fibroblasts
Author(s): 
Institution: 
  • Universidade Estadual Paulista (UNESP)
  • Malmö University
  • Universidade de São Paulo (USP)
  • Universidade Federal do Rio de Janeiro (UFRJ)
ISSN: 
1549-3296
Sponsorship: 
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Abstract: 
Implant topography is an important factor that influences many cell types. To understand the role of topography in the inflammatory events, we evaluated the response of human gingival fibroblasts (HGFs) by the release pattern of cytokines. HGFs were cultured on Ti discs for 24 and 48 h. Four different surface treatments were used: machining method (turned), blasting followed by an acid-etching method (BAE), oxidative nanopatterning (ON) method, and an association of blasting followed by an acid-etching plus oxidative nanopatterning (BAE+ON) method. Extracellular levels of IL-6, IL-8, transforming growth factor beta (TGF-beta), IL-4, and IL-10 were measured by enzyme-linked immunosorbant assay. Increased levels of IL-6 and IL-8 were observed in all surfaces after 24 h which decreased after 48 h. BAE, ON, and BAE+ON surfaces showed a reduction in IL-6 levels compared with the turned after 48 h (p < 0.05). on one hand, IL-8 production was lower in BAE+ON in comparison to the turned surface (p < 0.05). on the other hand, IL-4 showed increased levels with 48 h, which were significantly different between turned, BAE, and on surfaces, but not with BAE+ON. Additionally, TGF-beta and IL-10 production were not detected. This study indicates that nanotopography might be important in the modulation of the inflammatory response in cultured HGFs. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 100A:2629-2636, 2012.
Issue Date: 
1-Oct-2012
Citation: 
Journal of Biomedical Materials Research Part A. Hoboken: Wiley-blackwell, v. 100A, n. 10, p. 2629-2636, 2012.
Time Duration: 
2629-2636
Publisher: 
Wiley-Blackwell
Keywords: 
  • cytokines
  • inflammation
  • fibroblast
  • nanotopography
  • titanium
Source: 
http://dx.doi.org/10.1002/jbm.a.34200
URI: 
Access Rights: 
Acesso restrito
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/15922
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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