Effect of red and infrared low-level laser therapy in endodontic sealer on subcutaneous tissue

Abstract

This study evaluated the reactions of connective tissue after the implant of one endodontic sealer (Endofill) that was irradiated with low-level laser therapy (LLLT). Sixty mice were distributed into three Groups (n = 20): GI-the tubes filled with Endofill were implanted in the animals and were not irradiated with LLLT; GII-the tubes containing Endofill were implanted in the animals and then irradiated with red LLLT (InGaAlP, lambda = 685 nm, P = 35 mW, t = 58 s, D = 72 J/cm(2), E = 2 J, = 0.60 mm, continuous mode) and GIII-the tubes with Endofill were implanted and irradiated with infrared LLLT (AsGaAl, lambda = 830 nm, P = 50 mW, t = 40 s, D = 70 J/cm(2), E = 2 J, = 0.60 mm, continuous wave) both are semiconductor diode laser device. The animals were killed after 7 and 30 days. Series sections of 6 mu m thickness were obtained and stained with Hematoxylin-Eosin and Masson Trichrome. The data of the histopathological evaluation were submitted to Kruskal-Wallis and Dunn's tests at 5% significance level. At the 7th day: GI showed the presence of inflammation; GII and GIII reduced inflammation. At 30th day: GI showed low inflammation; GII and GII the absence of inflammation. It was possible show that LLLT reduced the irritating effect promoted by the Endofill, in the period of 7 days (p > 0.05). The tissue repair occurred in 30 days, regardless of the use of LLLT.