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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/18694
Title: 
KARYOTYPE and NUCLEOLUS ORGANIZER REGIONS of Ophisternon aenigmaticum (Teleostei: Synbranchiformes: Synbranchidae) FROM VENEZUELA
Author(s): 
Institution: 
  • Universidade Estadual Paulista (UNESP)
  • Universidade Estadual de Campinas (UNICAMP)
  • Universidade de São Paulo (USP)
ISSN: 
0378-1844
Sponsorship: 
  • Consejo de Investigacion, Universidad de Oriente, Venezuela
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Abstract: 
The first cytogenetic analysis by conventional Giemsa staining silver nitrate impregnation of nucleolus organizer regions (Ag-NOR), and fluorescence in situ hybridization (FISH) carried out on Ophisternon aenigmaticum from Margarita Island, Venezuela, is reported. Two cytotypes were found: cytotype C1, 2n=46, composed of 6 medium size metacentrics, 2 subtelocentrics and 38 acrocentrics; and cytotype C2, 2n=45, composed of one large metacentric plus 5 medium size metacentrics, I subtelocentric and 38 acrocentric chromosomes. In cytotype C1, major ribosomal DNA (rDNA) cistrons are located in the terminal position of the short arms of the first metacentric pair as determined by Ag-NORs and FISH with the 18S rDNA probe. In cytotype C2, silver staining revealed only one positive signal in a single chromosome of the pair 1 whereas FISH with the 18S rDNA probe hybridized in two different chromosomes: one coincident with the Ag-NOR (pair 1) and other in one acrocentric chromosome, suggesting the occurrence of a pen-centric inversion. In both cytotypes, FISH with 5S rDNA probe produced hybridization signals in subtelomeric position of the short arm of the chromosome pair N(o) 3. Chromosome evolution in Synbranchidae is discussed.
Issue Date: 
1-Mar-2011
Citation: 
Interciencia. Caracas: Interciencia, v. 36, n. 3, p. 229-233, 2011.
Time Duration: 
229-233
Publisher: 
Interciencia
Keywords: 
  • Chromosome Evolution
  • Chromosome Fusion
  • 18S DNA
  • Fish Chromosome
  • Pericentric Inversion
  • 5S rDNA
Source: 
http://www.redalyc.org/articulo.oa?id=33917977012
URI: 
Access Rights: 
Acesso aberto
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/18694
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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