Histological and Histochemical Analysis of the Fat Body of Rhinocricus padbergi (Diplopoda) Exposed to Contaminated Industrial Soil

Abstract

Diplopods are components of the edaphic fauna, which makes them suitable bioindicators of soil quality. Some characteristics of the fat body of diplopods make it suitable for ecotoxicological studies. This organ, composed of diffuse tissue, fills the body cavity; it presents intense metabolic activity associated with lipid, glycogen, protein, and uric acid storage, being also responsible for storage, neutralization, and excretion of substances that are not useful to the organism. The present study aimed to investigate whether the perivisceral fat body of Rhinocricus padbergi can be used as a target organ in ecotoxicological studies and to identify possible histological and histochemical biomarkers in this organ. Upon examining the perivisceral fat body of individuals of R. padbergi exposed to different concentrations of industrial soil contaminated with polycyclic aromatic hydrocarbons (Sigma 2,749.0 mg/kg) and metals (Sigma 40,355.8 mg/kg), we have found evidence that this organ is sensitive to environmental pollutants present in the soil. The loss of integrity of the cell boundary associated with cytoplasmatic disorganization and depletion in total proteins, neutral polysaccharides, calcium, and lipids can be considered stress biomarkers for R. padbergi.