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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/32395
Title: 
Assay of Serum Glutamic-Oxaloacetic Transaminase Using Malate Dehydrogenase Preparation Obtained from Streptomyces aureofaciens
Author(s): 
Institution: 
  • Universidade Estadual de Campinas (UNICAMP)
  • Universidade Estadual Paulista (UNESP)
ISSN: 
0003-2719
Abstract: 
A modified spectrophotometric method for serum glutamic-oxaloacetic transaminase (SGOT) assay was developed. A crude cell-free extract from Streptomyces aureofaciens which showed a high level of malate dehydrogenase (MDH) activity (E.C. 1.1.1.37) was used as the enzymatic indicator. The lyophilized microbial preparation was used without previous purification and was quite stable under refrigeration for one year. Serum sample assays using both the method utilizing the crude cell extract and an enzymatic commercial kit showed good correlation.
Issue Date: 
1-Jan-1995
Citation: 
Analytical Letters. New York: Marcel Dekker Inc., v. 28, n. 13, p. 2305-2316, 1995.
Time Duration: 
2305-2316
Publisher: 
Marcel Dekker Inc
Keywords: 
  • TRANSAMINASE
  • SGOT
  • MALATE DEHYDROGENASE
  • STREPTOMYCES
Source: 
http://dx.doi.org/10.1080/00032719508000374
URI: 
Access Rights: 
Acesso restrito
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/32395
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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