Please use this identifier to cite or link to this item:
http://acervodigital.unesp.br/handle/11449/39029
- Title:
- Properties of a polynucleotide synthesized by strain 74a of Neurospora crassa
- Universidade de São Paulo (USP)
- Universidade Estadual Paulista (UNESP)
- 0031-9422
- A polynucleotide (or a fragment of RNA) was purified to apparent homogeneity by HPLC from mycelium of the wild strain 74A of the mould Neurospora crassa, after growth on sucrose and in the presence of saturating amounts of inorganic phosphate (Pi) for 72 hr at 30 degrees. The M(r) was ca 20000 as determined by HPLC at pH 6.8. Polynucleotide synthesis ranged from 4.0 to 6.5 mu g polynucleotide per mg dry mycelium in mycelium of the wild strain 74A and the various phosphorus regulatory and structural mutant strains of the mould N. crassa. Kinetic data showed that the polynucleotide interacts with mycelial Pi-repressible alkaline phosphatase by inhibiting its p-nitrophenylphosphatase activity and by protecting the enzyme against thermal inactivation in the presence of high concentrations of ammonium sulphate.
- 1-Feb-1996
- Phytochemistry. Oxford: Pergamon-Elsevier B.V., v. 41, n. 2, p. 345-348, 1996.
- 345-348
- Elsevier B.V.
- Neurospora crassa
- fungi
- polynucleotide
- alkaline phosphatase
- RNA-binding proteins
- http://dx.doi.org/10.1016/0031-9422(95)00531-5
- Acesso restrito
- outro
- http://repositorio.unesp.br/handle/11449/39029
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.