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http://acervodigital.unesp.br/handle/11449/39440
- Title:
- Isolation and characterisation of cycloheximide sensitive mutants of Aspergillus nidulans
- Universidade de São Paulo (USP)
- Universidade Estadual Paulista (UNESP)
- 0172-8083
- Aspergillus nidulans is a non-pathogenic fungus with well-developed genetics which provides an excellent model system for studying different aspects of drug resistance in filamentous fungi. As a preliminary step to characterizing genes that confer pleiotropic drug resistance in Aspergillus, we isolated cycloheximide-sensitive mutants of A. nidulans, which is normally resistant to this: drug. The rationale for this approach is to identify gents whose products are important for drug resistance by analysing mutations that alter the resistance/sensitivity status of the cell. Fifteen cycloheximide-sensitive (named scy for sensitive to cycloheximide) mutants of A, nidulans were isolated and genetically characterised. Each scy mutant was crossed with the wild-type strain and five of the crosses gave 50% cycloheximide-sensitive progeny suggesting that they carry a single mutation required for cycloheximide sensitivity. We examined ten sep mutants for resistance/sensitivity to other drugs or stress agents with different and/or the same mechanism of action, Sis of these mutants exhibited other altered resistance/sensitivity phenotypes which were linked to the cycloheximide sensitivity, These six mutants were analyzed by pairwise crosses and found to represent six linkage groups, named scyA-F. One of the mutants showed fragmentation of its vacuolar system and, in addition, its growth was osmotic, low-pi-II and oxidative-stress sensitive.
- 1-Jan-1998
- Current Genetics. New York: Springer, v. 33, n. 1, p. 60-69, 1998.
- 60-69
- Springer
- cycloheximide-sensitive mutants
- Aspergillus nidulans
- pleitropic drug resistance
- vacuoles
- http://dx.doi.org/10.1007/s002940050309
- Acesso restrito
- outro
- http://repositorio.unesp.br/handle/11449/39440
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