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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/39440
Title: 
Isolation and characterisation of cycloheximide sensitive mutants of Aspergillus nidulans
Author(s): 
Institution: 
  • Universidade de São Paulo (USP)
  • Universidade Estadual Paulista (UNESP)
ISSN: 
0172-8083
Abstract: 
Aspergillus nidulans is a non-pathogenic fungus with well-developed genetics which provides an excellent model system for studying different aspects of drug resistance in filamentous fungi. As a preliminary step to characterizing genes that confer pleiotropic drug resistance in Aspergillus, we isolated cycloheximide-sensitive mutants of A. nidulans, which is normally resistant to this: drug. The rationale for this approach is to identify gents whose products are important for drug resistance by analysing mutations that alter the resistance/sensitivity status of the cell. Fifteen cycloheximide-sensitive (named scy for sensitive to cycloheximide) mutants of A, nidulans were isolated and genetically characterised. Each scy mutant was crossed with the wild-type strain and five of the crosses gave 50% cycloheximide-sensitive progeny suggesting that they carry a single mutation required for cycloheximide sensitivity. We examined ten sep mutants for resistance/sensitivity to other drugs or stress agents with different and/or the same mechanism of action, Sis of these mutants exhibited other altered resistance/sensitivity phenotypes which were linked to the cycloheximide sensitivity, These six mutants were analyzed by pairwise crosses and found to represent six linkage groups, named scyA-F. One of the mutants showed fragmentation of its vacuolar system and, in addition, its growth was osmotic, low-pi-II and oxidative-stress sensitive.
Issue Date: 
1-Jan-1998
Citation: 
Current Genetics. New York: Springer, v. 33, n. 1, p. 60-69, 1998.
Time Duration: 
60-69
Publisher: 
Springer
Keywords: 
  • cycloheximide-sensitive mutants
  • Aspergillus nidulans
  • pleitropic drug resistance
  • vacuoles
Source: 
http://dx.doi.org/10.1007/s002940050309
URI: 
Access Rights: 
Acesso restrito
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/39440
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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