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http://acervodigital.unesp.br/handle/11449/39624
- Title:
- Cytotoxicity, genotoxicity and antimutagenicity of hexane extracts of Agaricus blazei determined in vitro by the comet assay and CHO/HGPRT gene mutation assay
- Universidade Estadual de Londrina (UEL)
- Universidade Estadual Paulista (UNESP)
- Universidade Federal de São Carlos (UFSCar)
- 0887-2333
- Agaricus blazei Murrill ss. Heinem, known as the sun mushroom or himematsutake, is a basidiomycete native to Brazil, which is popular for its medicinal properties. The aim of this study was to test hexane extracts (one fraction and its four sub-fractions) of A. blazei for bioactivity in cultured mammalian cells (CHO-K1). The comet assay, the colony forming assay (CFA) and CHO/HGPRT gene mutation assay were used respectively to determine genotoxicity, cytotoxicity and antimutagenicity of these extracts at different concentrations. The cells were incubated in culture medium and treated for 3 h according to the standard protocol for each assay. The DNA damage-inducing agent ethylmethane sulfonate (EMS) was utilized as the positive control and also in combination with extracts to test for a protective effect. Statistical analysis of the data was performed using analysis of variance (ANOVA) and Tukey's test. A relationship between cytotoxicity and genotoxicity could be established and two extracts EH6B and EH6D showed a protective tendency, while the others did not, with the primary extract EH6 causing the most substantial damage to genetic material. These findings warrant more in-depth studies of the active principles of this mushroom. (c) 2005 Elsevier Ltd. All rights reserved.
- 1-Jun-2005
- Toxicology In Vitro. Oxford: Pergamon-Elsevier B.V., v. 19, n. 4, p. 533-539, 2005.
- 533-539
- Elsevier B.V.
- Agaricus blazei
- CHO-K1 cells
- comet assay
- colony forming assay
- CHO/HGPRT test
- ethylmethane sulfonate
- http://dx.doi.org/10.1016/j.tiv.2004.12.005
- Acesso restrito
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- http://repositorio.unesp.br/handle/11449/39624
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