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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/40467
Title: 
Isolation and characterization of 15 polymorphic microsatellites in the Plethodontid salamander Ensatina eschscholtzii
Author(s): 
Institution: 
  • Univ Calif Berkeley
  • Univ Porto
  • Universidade Estadual Paulista (UNESP)
  • Univ Calif Los Angeles
ISSN: 
1755-098X
Sponsorship: 
NSF
Sponsorship Process Number: 
NSF: DEB 0641078
Abstract: 
We developed 15 new polymorphic microsatellites for the plethodontid salamander Ensatina eschscholtzii. Loci were isolated from a genomic library from Ensatina eschscholtzii xanthoptica enriched for (AAAG)(n) repetitive elements. The number of alleles per locus ranged from 4 to 20 (mean 9) in the sampled population. Observed heterozygosity ranged from 0.37 to 1. None of the loci deviated from Hardy-Weinberg equilibrium or showed significant linkage disequilibrium after a Bonferroni correction for multiple comparisons. All loci amplified in the six other subspecies of the Ensatina eschscholtzii complex. These new markers will prove useful in measuring gene flow and population structure as well as patterns of mating and sperm use in Ensatina.
Issue Date: 
1-May-2009
Citation: 
Molecular Ecology Resources. Malden: Wiley-blackwell Publishing, Inc, v. 9, n. 3, p. 966-969, 2009.
Time Duration: 
966-969
Publisher: 
Wiley-Blackwell Publishing, Inc
Keywords: 
  • Ensatina
  • microsatellite
  • Plethodontidae
  • plethodontid
  • ring species
  • salamander
Source: 
http://dx.doi.org/10.1111/j.1755-0998.2009.02518.x
URI: 
Access Rights: 
Acesso restrito
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/40467
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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