A Preliminary and Qualitative Metallomics Study of Mercury in the Muscle of Fish from Amazonas, Brazil

Moraes, Paula M.; Santos, Felipe A.; Padilha, Cilene C. F.; Vieira, Jose C. S.; Zara, Luiz F.; Padilha, Pedro de M.

Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/40765

Title:

A Preliminary and Qualitative Metallomics Study of Mercury in the Muscle of Fish from Amazonas, Brazil

Author(s):

Institution:

Universidade Estadual Paulista (UNESP)
Universidade de Brasília (UnB)
Universidade Estadual de Campinas (UNICAMP)

ISSN:

0163-4984

Sponsorship:

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Sponsorship Process Number:

FAPESP: 10/51332-5
FAPESP: 09/54856-8

Abstract:

This paper presents preliminary findings for a metallomics study of mercury in the muscle of the fish species from Amazonas, Brazil, after protein separation by two-dimensional polyacrylamide gel electrophoresis and subsequent evaluation of mercury by synchrotron radiation X-ray fluorescence. The fluorescence spectra revealed mercury in two protein spots. The mercury-containing protein spots showed molecular weights of 20.8 +/- 0.7 and 19.8 +/- 0.5 kDa and isoelectric points of 5.6 +/- 0.2 and 7.5 +/- 0.3, respectively.

Issue Date:

1-Dec-2012

Citation:

Biological Trace Element Research. Totowa: Humana Press Inc, v. 150, n. 1-3, p. 195-199, 2012.

Time Duration:

195-199

Publisher:

Humana Press Inc

Keywords:

Metallomics study
Mercury in fish
2D PAGE
SR XRF

Source:

http://dx.doi.org/10.1007/s12011-012-9502-x

URI:

Access Rights:

Acesso restrito

Type:

outro

Source:

http://repositorio.unesp.br/handle/11449/40765

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