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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/42007
Title: 
Identification of psoralen loaded PLGA microspheres in rat skin by light microscopy
Author(s): 
Institution: 
  • Universidade Federal de Uberlândia (UFU)
  • Fac Ciencias Farmaceut Ribeirao Preto
  • Universidade Estadual Paulista (UNESP)
ISSN: 
0968-4328
Abstract: 
Drug delivery systems involving the use of polymers are widely studied and discovery of biocompatible polymers has become the focus of research in this area. Psoralen loaded poly(DL-lactide-co-glycolide) (PLGA) microspheres to be used in PUVA therapy (psoralen and UVA irradiation (ultraviolet A, 320-400 nm) of psoriasis were identified in paraffin sections by histological analysis. The psoralen loaded PLGA microspheres were prepared using the solvent evaporation technique. They were spherical and possessed an external smooth surface as observed by scanning electron microscopy (SEM) analysis. This study describes a modification in the routine preparation of microsphere samples for examination by light microscopy. The changes involved fixative agents and/or stains allowing the identification of microspheres containing a non-fluorescent material. The preservation and identification of microspheres in tissues for histological processing in paraffin was greatly improved by these modifications as proven by our results. (c) 2007 Elsevicr Ltd. All rights reserved.
Issue Date: 
1-Jan-2008
Citation: 
Micron. Oxford: Pergamon-Elsevier B.V. Ltd, v. 39, n. 1, p. 40-44, 2008.
Time Duration: 
40-44
Publisher: 
Pergamon-Elsevier B.V. Ltd
Keywords: 
  • drug delivery
  • light microscopy
  • microspheres
  • PLGA
  • psoralen
  • rat skin
  • irradiation
  • stain
Source: 
http://dx.doi.org/10.1016/j.micron.2007.06.015
URI: 
Access Rights: 
Acesso restrito
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/42007
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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