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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/64733
Title: 
Evaluation of an indirect ELISA method for the detection of chicken antibodies against infectious bronchitis virus
Author(s): 
Institution: 
  • MEC
  • Universidade Estadual Paulista (UNESP)
  • SOLVAY Saúde Animal
ISSN: 
0001-3714
Sponsorship: 
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Abstract: 
An indirect ELISA method was developed and applied to detect antibodies against infectious bronchitis virus (IBV). Three hundred serum samples collected from vaccinated and non-vaccinated chickens under field conditions were tested. The ELISA results were compared with antibody titers determined by a serum neutralization assay (SN). Good coefficients of correlation (r2 = 0.750) and of agreement (80%) were recorded and the ELISA reproducibility was high. The cutoff point between those techniques corresponded to ELISA level 2. It is concluded that our indirect ELISA method can advantageously replace the conventional SN test, allowing a good follow-up of IBV post-vaccination antibodies due to its convenience and applicability compared to the other assay.
Issue Date: 
1-Jan-1996
Citation: 
Revista de Microbiologia, v. 27, n. 1, p. 64-69, 1996.
Time Duration: 
64-69
Keywords: 
  • ELISA
  • Infectious Bronchitis Virus (IBV)
  • Post-vaccine serological monitoring
URI: 
Access Rights: 
Acesso restrito
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/64733
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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