Micropropagação de babosa (Aloe vera L.)

Abstract

The demand for hiah aenetic and fit sanitary quality plantlets of medicinal plants is increasing. The babosa (Aloe vera L.) is propagated conventionally through lateral buds, which is a slowly, very expensive and low income practice. Thus, the objective of this work was the development of A. vera micropropagation protocol. Meristems of A. vera were submitted to asepsis tests, varying the time of immersion in 3% NaOCI solution, 70% alcohol solution and after were submitted in vitro multiplication with effect of combinations and concentrations of vegetal regulators BA (6-Benzylaminopurine), KIN (Kinetin), IAA (lndole-3-acetic acid) and NAA (á Naphthyl acetic acid) supplemented in the MS media culture during 180 days (6 subcultures). For the initial establishment, the best disinfestations treatment was the meristem immersion for 20 minutes in 3% NaOCI solution + 1 minute in 70% alcohol solution, repeating that sequence in the board and after in the laminar flow. In relation to in vitro multiplication, the best results were the use of MS media culture with out vegetal regulators in the first subculture (30 days), supplemented with 2.85 mmol L -1 of IAA + 4.44 mmol L -1 of BA in the second subculture (60 days), 2.85 mmol L -1 of IAA+ 8.88 mmol L -1 of BA in the third subculture (90 days), 2.85 mmol L -1 of IAA+22.2 mmol L -1 of BA in the fourth subculture (120 days), 4.44 mmol/L of BA in the fifth subculture (150 days) and again removing the vegetal regulators in the sixth subculture (180 days), reaching an average of 201 new plantlets of A. vera in a period of 6 months.