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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/69947
Title: 
The effects of male age on sperm DNA damage in an infertile population
Author(s): 
Institution: 
  • CPDP-Paulista Centre for Diagnosis and Research
  • Centre for Human Reproduction Prof. Franco Junior
  • Universidade Estadual Paulista (UNESP)
ISSN: 
1472-6483
Abstract: 
The objective was to investigate the influence of age on sperm DNA damage. Semen samples were collected from 508 men in an unselected group of couples attending infertility investigation and treatment. DNA fragmentation in spermatozoa was measured by TdT (terminal deoxynucleotidyl transferase)-mediated dUTP nick-end labelling (TUNEL) assay; at least 200 spermatozoa in randomly selected areas of microscope slides were evaluated using a fluorescent microscope and the percentage of TUNEL positive spermatozoa was determined. The number of cells with red fluorescence (TUNEL positive) was expressed as a percentage of the total sample [DNA fragmentation index (DFI)]. Age was treated as a continuous variable for regression and correlation analysis. The following male age groups were used: Group I: ≤35 years, Group II: 36-39 years, and Group III: ≥40 years. DFI was significantly lower in Group I than in Group II (P = 0.034) or III (P = 0.022). There was no difference in DFI between Groups II and III. In addition, regression analysis demonstrated a significant increase in sperm DFI with age (P = 0.02). TUNEL assay clearly demonstrates an increase in sperm DNA damage with age. © 2007 Published by Reproductive Healthcare Ltd.
Issue Date: 
1-Nov-2007
Citation: 
Reproductive BioMedicine Online, v. 15, n. 5, p. 514-519, 2007.
Time Duration: 
514-519
Keywords: 
  • Age
  • DNA damage
  • Male infertility
  • Spermatozoa
  • TUNEL
  • DNA
  • DNA fragment
  • adult
  • age
  • asthenospermia
  • controlled study
  • correlation analysis
  • fluorescence microscopy
  • human
  • major clinical study
  • male
  • male infertility
  • nick end labeling
  • oligospermia
  • regression analysis
  • semen analysis
  • spermatozoon
  • Adult
  • Aged
  • Aging
  • DNA Damage
  • Humans
  • In Situ Nick-End Labeling
  • Infertility, Male
  • Male
  • Middle Aged
Source: 
http://dx.doi.org/10.1016/S1472-6483(10)60382-3
URI: 
Access Rights: 
Acesso restrito
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/69947
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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