Identification of ellagic acid derivatives in methanolic extracts from Qualea species

Abstract

The methanolic extract from the barks of the medicinal plant Qualea parviflora (Vochysiaceae) was fractionated by column chromatography over silica gel followed by gel permeation over Sephadex LH-20 to give 3,3′-di-O- methylellagic acid-4-O-β-D-glucopyranoside (1), 3-O-methylellagic acid-4′-O-α-L-rhamnopyranoside (2), 3,3′,4-tri-O-methylellagic acid-4′-O-β-D-glucopyranoside (3), and 3,3′-di-O-methylellagic acid (4), together with triterpenes and saponins. We also performed comparative analyses among this species and Q. grandiflora and Q. multiflora using high-pressure liquid chromatography. The biological assays showed that, when compared to the standard ellagic acid, compounds 1-4 are less cytotoxic but have a lower capacity of stimulating murine peritoneal macrophages to release nitric oxide and tumoural-α necrose factor. © 2008 Verlag der Zeitschrift für Naturforschung.