Interaction of Bacillus thuringiensis Cry1 and Vip3A proteins with Spodoptera frugiperda midgut binding sites

Sena, Janete A. D.; Hernández-Rodríguez, Carmen Sara; Ferré, Juan

Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/70965

Title:

Interaction of Bacillus thuringiensis Cry1 and Vip3A proteins with Spodoptera frugiperda midgut binding sites

Author(s):

Institution:

Universidad de Valencia
Facultad de CC. Biológicas
Universidade Estadual Paulista (UNESP)

ISSN:

0099-2240
1098-5336

Abstract:

Vip3Aa, Vip3Af, Cry1Ab, and Cry1Fa were tested for their toxicities and binding interactions. Vip3A proteins were more toxic than Cry1 proteins. Binding assays showed independent specific binding sites for Cry1 and Vip3A proteins. Cry1Ab and Cry1Fa competed for the same binding sites, whereas Vip3Aa competed for those of Vip3Af. Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Issue Date:

1-Apr-2009

Citation:

Applied and Environmental Microbiology, v. 75, n. 7, p. 2236-2237, 2009.

Time Duration:

2236-2237

Keywords:

Bacillus thuringiensis
Binding assays
Binding interactions
Specific bindings
Spodoptera frugiperda
Bacteriology
Binding energy
Proteins
Binding sites
Bacillus thuringiensis toxin
bacterial toxin
Cry1Ab toxin
cry1fa protein
unclassified drug
vip3aa protein
vip3af protein
bacterium
caterpillar
protein
toxicity
bacterial strain
binding site
bioassay
Lepidoptera
midgut
nonhuman
protein analysis
protein expression
protein protein interaction
Animals
Bacterial Proteins
Endotoxins
Gastrointestinal Tract
Hemolysin Proteins
Larva
Lethal Dose 50
Protein Binding
Spodoptera

Source:

http://dx.doi.org/10.1128/AEM.02342-08

URI:

Access Rights:

Acesso restrito

Type:

outro

Source:

http://repositorio.unesp.br/handle/11449/70965

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