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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/71585
Title: 
Occurrence and expression of p53 suppressor gene and c-Myc oncogene in dog eyelid tumors
Author(s): 
Institution: 
Universidade Estadual Paulista (UNESP)
ISSN: 
  • 1463-5216
  • 1463-5224
Abstract: 
Purpose: To detect the occurrence and expression of the suppressor gene p53 and of the oncogene c-Myc in eyelid tumors of dogs using the PCR, RT-PCR, PCR-ELISA and RT-PCR-ELISA techniques. These genes have not been described in dog eyelid tumors before. Methods: Nine samples of eyelid or third eyelid epithelial tumors were obtained from the archives of the Department of Veterinary Pathology. Tumor diagnosis was confirmed by evaluation of hematoxylin-eosin stained sections, and immunohistochemistry for cytokeratin AE1/AE3 and vimentin V9. A canine mammary tumor was used for positive control. Agarose gel electrophoresis, PCR-ELISA and RT-PCR-ELISA were used to detect p53 and c-Myc genes. Results: The occurrence of p53 was detected in most of the eyelid tumors and third eyelid tumors studied (88.8%, n = 8) and was expressed in 75% of the positive samples, as indicated by ELISA. The c-Myc gene was found in 77.7% (n = 7) of the samples and was expressed in eight samples. Conclusions: Eyelid and third eyelid tumors of dogs express both the p53 and the c-Myc genes as shown by PCR and RT-PCR. However, PCR ELISA and RT-PCR ELISA were more efficient in assessing occurrence and expression of these genes because they identified amplified products that were not detected by agarose gel electrophoresis. © 2010 American College of Veterinary Ophthalmologists.
Issue Date: 
1-Mar-2010
Citation: 
Veterinary Ophthalmology, v. 13, n. 2, p. 69-75, 2010.
Time Duration: 
69-75
Keywords: 
  • C-Myc
  • Dog
  • ELISA
  • Eyelid tumors
  • P53
  • PCR
  • DNA
  • Myc protein
  • protein p53
  • RNA
  • animal
  • animal disease
  • dog
  • dog disease
  • eyelid tumor
  • gene expression regulation
  • genetics
  • metabolism
  • physiology
  • Animals
  • Dog Diseases
  • Dogs
  • Eyelid Neoplasms
  • Gene Expression Regulation, Neoplastic
  • Proto-Oncogene Proteins c-myc
  • Tumor Suppressor Protein p53
  • Canis familiaris
Source: 
http://dx.doi.org/10.1111/j.1463-5224.2009.00753.x
URI: 
Access Rights: 
Acesso restrito
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/71585
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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