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http://acervodigital.unesp.br/handle/11449/71585
- Title:
- Occurrence and expression of p53 suppressor gene and c-Myc oncogene in dog eyelid tumors
- Universidade Estadual Paulista (UNESP)
- 1463-5216
- 1463-5224
- Purpose: To detect the occurrence and expression of the suppressor gene p53 and of the oncogene c-Myc in eyelid tumors of dogs using the PCR, RT-PCR, PCR-ELISA and RT-PCR-ELISA techniques. These genes have not been described in dog eyelid tumors before. Methods: Nine samples of eyelid or third eyelid epithelial tumors were obtained from the archives of the Department of Veterinary Pathology. Tumor diagnosis was confirmed by evaluation of hematoxylin-eosin stained sections, and immunohistochemistry for cytokeratin AE1/AE3 and vimentin V9. A canine mammary tumor was used for positive control. Agarose gel electrophoresis, PCR-ELISA and RT-PCR-ELISA were used to detect p53 and c-Myc genes. Results: The occurrence of p53 was detected in most of the eyelid tumors and third eyelid tumors studied (88.8%, n = 8) and was expressed in 75% of the positive samples, as indicated by ELISA. The c-Myc gene was found in 77.7% (n = 7) of the samples and was expressed in eight samples. Conclusions: Eyelid and third eyelid tumors of dogs express both the p53 and the c-Myc genes as shown by PCR and RT-PCR. However, PCR ELISA and RT-PCR ELISA were more efficient in assessing occurrence and expression of these genes because they identified amplified products that were not detected by agarose gel electrophoresis. © 2010 American College of Veterinary Ophthalmologists.
- 1-Mar-2010
- Veterinary Ophthalmology, v. 13, n. 2, p. 69-75, 2010.
- 69-75
- C-Myc
- Dog
- ELISA
- Eyelid tumors
- P53
- PCR
- DNA
- Myc protein
- protein p53
- RNA
- animal
- animal disease
- dog
- dog disease
- eyelid tumor
- gene expression regulation
- genetics
- metabolism
- physiology
- Animals
- Dog Diseases
- Dogs
- Eyelid Neoplasms
- Gene Expression Regulation, Neoplastic
- Proto-Oncogene Proteins c-myc
- Tumor Suppressor Protein p53
- Canis familiaris
- http://dx.doi.org/10.1111/j.1463-5224.2009.00753.x
- Acesso restrito
- outro
- http://repositorio.unesp.br/handle/11449/71585
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