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http://acervodigital.unesp.br/handle/11449/7563
- Title:
- p-iodophenol-enhanced luminol chemiluminescent assay applied to discrimination between acute lymphoblastic and minimally differentiated acute myeloid (FAB-M0) or acute megakaryoblastic (FAB-M7) leukemias
- Universidade Estadual Paulista (UNESP)
- Universidade de São Paulo (USP)
- 1466-4860
- Introduction: In this report, we propose the application of the p-iodophenol-enhanced luminol chemiluminescent technique to the determination of peroxidase (myeloperoxidase and/or platelet peroxidase) activity in blasts of minimally differentiated acute myeloblastic leukemia (AML-M0) and acute megakaryoblastic leukemia (AML-M7).Methods: the frozen blast cells from 29 patients were thawed and submitted to the optimized protocol.Results: All cases of AML-M7 and AML-M0 exhibited integrated light emission greater than 73 (10(2) mV x s), which was the arbitrary cutoff point set for the discrimination between AML and acute lymphoblastic leukemia (ALL) (mean + 3 x s.d. of ALL samples, n = 10). In addition, five out of seven cases of AML-M0 showed results above the Cutoff point.Conclusion: This highly sensitive enhanced chemiluminescent technique may be applied to discriminate between ALL and AML-M7 or AML-M1 cases, and most AML-M0 cases. It is very simple, cheap and easy to perform compared to other procedures used to measure MPO activity in AML-leukemias including AML-M7 and AML-M0.
- 1-Jan-2004
- Hematology Journal. London: Nature Publishing Group, v. 5, n. 6, p. 496-499, 2004.
- 496-499
- Nature Publishing Group
- acute myeloid leukemia (AML)
- acute lymphoblastic leukemia (ALL)
- chemiluminescence
- luminol
- p-iodophenol
- myeloperoxidase (MPO)
- platelet peroxidase (PPO)
- http://dx.doi.org/10.1038/sj.thj.6200562
- Acesso restrito
- outro
- http://repositorio.unesp.br/handle/11449/7563
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