Please use this identifier to cite or link to this item:
http://acervodigital.unesp.br/handle/11449/75669
- Title:
- Peripheral kappa and delta opioid receptors are involved in the antinociceptive effect of crotalphine in a rat model of cancer pain
- Instituto Butantan
- Universidade de São Paulo (USP)
- Universidade Estadual Paulista (UNESP)
- University of Toyama
- 0091-3057
- 1873-5177
- Cancer pain is an important clinical problem and may not respond satisfactorily to the current analgesic therapy. We have characterized a novel and potent analgesic peptide, crotalphine, from the venom of the South American rattlesnake Crotalus durissus terrificus. In the present work, the antinociceptive effect of crotalphine was evaluated in a rat model of cancer pain induced by intraplantar injection of Walker 256 carcinoma cells. Intraplantar injection of tumor cells caused the development of hyperalgesia and allodynia, detected on day 5 after tumor cell inoculation. Crotalphine (6 μg/kg), administered p.o., blocked both phenomena. The antinociceptive effect was detected 1 h after treatment and lasted for up to 48 h. Intraplantar injection of nor-binaltorphimine (50 g/paw), a selective antagonist of κ-opioid receptors, antagonized the antinociceptive effect of the peptide, whereas N,N-diallyl-Tyr-Aib-Phe-Leu (ICI 174,864, 10 μg/paw), a selective antagonist of δ-opioid receptors, partially reversed this effect. On the other hand, D-Phe-Cys-Tyr-D-Trp-Orn-Thr-Pen-Thr amide (CTOP, 20 g/paw), an antagonist of μ-opioid receptors, did not modify crotalphine-induced antinociception. These data indicate that crotalphine induces a potent and long lasting opioid-mediated antinociception in cancer pain. © 2013 Elsevier Inc.
- 17-Jun-2013
- Pharmacology Biochemistry and Behavior, v. 109, p. 1-7.
- 1-7
- Antinociception
- Cancer pain
- Crotalphine
- Opioid receptors
- Walker 256 carcinoma cells
- antinociceptive agent
- crotalphine
- delta opiate receptor
- kappa opiate receptor
- morphine
- unclassified drug
- animal experiment
- animal model
- antinociception
- cancer growth
- cancer pain
- drug effect
- drug efficacy
- locomotion
- male
- nonhuman
- pain threshold
- priority journal
- rat
- http://dx.doi.org/10.1016/j.pbb.2013.04.012
- Acesso restrito
- outro
- http://repositorio.unesp.br/handle/11449/75669
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.