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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/75669
Title: 
Peripheral kappa and delta opioid receptors are involved in the antinociceptive effect of crotalphine in a rat model of cancer pain
Author(s): 
Institution: 
  • Instituto Butantan
  • Universidade de São Paulo (USP)
  • Universidade Estadual Paulista (UNESP)
  • University of Toyama
ISSN: 
  • 0091-3057
  • 1873-5177
Abstract: 
Cancer pain is an important clinical problem and may not respond satisfactorily to the current analgesic therapy. We have characterized a novel and potent analgesic peptide, crotalphine, from the venom of the South American rattlesnake Crotalus durissus terrificus. In the present work, the antinociceptive effect of crotalphine was evaluated in a rat model of cancer pain induced by intraplantar injection of Walker 256 carcinoma cells. Intraplantar injection of tumor cells caused the development of hyperalgesia and allodynia, detected on day 5 after tumor cell inoculation. Crotalphine (6 μg/kg), administered p.o., blocked both phenomena. The antinociceptive effect was detected 1 h after treatment and lasted for up to 48 h. Intraplantar injection of nor-binaltorphimine (50 g/paw), a selective antagonist of κ-opioid receptors, antagonized the antinociceptive effect of the peptide, whereas N,N-diallyl-Tyr-Aib-Phe-Leu (ICI 174,864, 10 μg/paw), a selective antagonist of δ-opioid receptors, partially reversed this effect. On the other hand, D-Phe-Cys-Tyr-D-Trp-Orn-Thr-Pen-Thr amide (CTOP, 20 g/paw), an antagonist of μ-opioid receptors, did not modify crotalphine-induced antinociception. These data indicate that crotalphine induces a potent and long lasting opioid-mediated antinociception in cancer pain. © 2013 Elsevier Inc.
Issue Date: 
17-Jun-2013
Citation: 
Pharmacology Biochemistry and Behavior, v. 109, p. 1-7.
Time Duration: 
1-7
Keywords: 
  • Antinociception
  • Cancer pain
  • Crotalphine
  • Opioid receptors
  • Walker 256 carcinoma cells
  • antinociceptive agent
  • crotalphine
  • delta opiate receptor
  • kappa opiate receptor
  • morphine
  • unclassified drug
  • animal experiment
  • animal model
  • antinociception
  • cancer growth
  • cancer pain
  • drug effect
  • drug efficacy
  • locomotion
  • male
  • nonhuman
  • pain threshold
  • priority journal
  • rat
Source: 
http://dx.doi.org/10.1016/j.pbb.2013.04.012
URI: 
Access Rights: 
Acesso restrito
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/75669
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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