You are in the accessibility menu

Please use this identifier to cite or link to this item:
Estratégias de inibição, mecanismos moleculares e interações intermoleculares em complexos macromoleculares
Murakami, Mario Tyago
Universidade Estadual Paulista (UNESP)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
This work presents some features of essential biological processes such as the haemostatic system, integrity of biological membranes and thermostability of proteins. Crystallographic, spectroscopic and in silico tools have been used to obtain information at the molecular level of macromolecular complexes, action mechanisms and inhibition pathways. Worms, snakes, ticks, leeches and spiders produce a variety of proteins, which interfere in the regulation of these systems. Different toxins isolated from these organisms were characterized providing necessary information for the development of a new anti-myonecrotic molecule and reveal a new factor Xa exosite that is important for macromolecular substrates recognition and inhibition. The first crystal structure of a member of the sphingomyelinases D family was determined by the quick cryo-soaking technique and the catalytic mechanism was proposed, which involves a magnesium-binding site and two catalytic histidines. An alternative activation of the protein C pathway that does not require thrombomodulin was structurally characterized and revealed the dual role of the elestrotatic surface charge around the active site and the three strategically positioned carbohydrate moieties in the approach, recognition and activation of protein C.
Issue Date: 
MURAKAMI, Mario Tyago. Estratégias de inibição, mecanismos moleculares e interações intermoleculares em complexos macromoleculares. 2006. 152 f. Tese (doutorado) - Universidade Estadual Paulista, Instituto de Biociências, Letras e Ciências Exatas, 2006.
Time Duration: 
152 f. : il.
Universidade Estadual Paulista (UNESP)
  • Proteínas - Estrutura
  • Raios X - Difração
  • Cristalografia de raio X
  • Sangue - Coagulação
  • Difração de raios X
  • Coagulação sanguínea
  • Processos biológicos
  • Mecanismos de ação e inibição
  • X-ray diffraction
  • Biological processes
  • Action mechanisms and inhibition
Access Rights: 
Acesso aberto
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.