You are in the accessibility menu

Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorGrillo, Renato-
dc.contributor.authorDe Melo, Nathalie F. S.-
dc.contributor.authorDe Araujo, Daniele R.-
dc.contributor.authorDe Paula, Eneida-
dc.contributor.authorDias Filho, Newton L.-
dc.contributor.authorRosa, Andre H.-
dc.contributor.authorFraceto, Leonardo F.-
dc.identifier.citationLatin American Journal of Pharmacy. La Plata: Colegio Farmaceuticos Provincia de Buenos Aires, v. 28, n. 3, p. 393-399, 2009.-
dc.description.abstractBenzocaine (BZC) is an ester-type local anesthetic used mainly in topical, dermal and mucosal formulations. The present work consists of the development and validation of analytical methodology for evaluation of benzocaine content in the micro and nanoparticles produced by biodegradable polymers (polyhydroxybutyrate-co-hydroxyvalerate and poly-(L-lactide) by HPLC. The validation was done using the reversed-phase C18 column, using a mobile phase consisting of acetonitrile/water 50:50 (v/v), flow rate of 1.5 mL/min and UV-vis detector at 285 nm. The results here obtained showed that the analytical methodology is accurate, reproducible,robust and linear over the molar range concentration of 10-100 mu M of benzocaine. The limit of quantification and detection was 13.06 mu M and 3.92 mu M, respectively. The encapsulation efficiency of benzocaine In PHBV microspheres and PLA nanocapsule were 40% and 70%, respectively. The method developed was applied in the analysis of benzocaine in micro and nanoparticle systems and showed to be efficient, yielding good results. Here, this method was used to evaluate the encapsulation efficiency of benzocaine and will be used in next studies with different micro/nanoparticle formulations.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.description.sponsorshipFundação para o Desenvolvimento da UNESP (FUNDUNESP)-
dc.publisherColegio Farmaceuticos Provincia de Buenos Aires-
dc.sourceWeb of Science-
dc.subjectLocal anestheticen
dc.titleValidation of an HPLC Method for quantitative Determination of Benzocaine in PHBV-Microparticles and PLA-Nanoparticlesen
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)-
dc.contributor.institutionUniversidade Federal do ABC (UFABC)-
dc.description.affiliationUniv Estadual Paulista, Dept Engenharia Ambiental, BR-18087180 São Paulo, Brazil-
dc.description.affiliationUniv Estadual Campinas, Dept Bioquim, Inst Biol, Campinas, SP, Brazil-
dc.description.affiliationUniversidade Federal do ABC (UFABC), Ctr Ciencias Humana & Nat, Santo Andre, SP, Brazil-
dc.description.affiliationUniv Estadual Paulista, Dept Quim & Fis, São Paulo, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Dept Engenharia Ambiental, BR-18087180 São Paulo, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Dept Quim & Fis, São Paulo, Brazil-
dc.description.sponsorshipIdFAPESP: 07/00127-0-
dc.description.sponsorshipIdFAPESP: 06/00121-9-
dc.description.sponsorshipIdFAPESP: 08/01222-9-
dc.description.sponsorshipIdFAPESP: 06/00121-0-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofLatin American Journal of Pharmacy-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.