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dc.contributor.authorTeresa Federici, Maria-
dc.contributor.authorMarcondes, Jackson A.-
dc.contributor.authorPicchi, Simone Cristina-
dc.contributor.authorStuchi, Eduardo S.-
dc.contributor.authorFadel, Andre Luiz-
dc.contributor.authorLaia, Marcelo L.-
dc.contributor.authorLemos, Manoel Victor F.-
dc.contributor.authorMacedo Lemos, Eliana Gertrudes-
dc.identifier.citationElectronic Journal of Biotechnology. Valparaiso: Univ Catolica de Valparaiso, v. 15, n. 3, p. 33, 2012.-
dc.description.abstractXylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels.en
dc.description.sponsorshipMinistry of Culture of Uruguay through the Program for Technical Development (PDT)-
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
dc.description.sponsorshipNational Institute of Agronomic Research of Uruguay (INIA)-
dc.publisherUniversidade Católica de Valparaíso-
dc.sourceWeb of Science-
dc.subjectXylella fastidiosaen
dc.titleXylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysisen
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
dc.contributor.institutionEstacao Expt Citricultura-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionUniv Fed Vales Jequitinhonha & Mucuri-
dc.contributor.institutionAgência Paulista de Tecnologia dos Agronegócios (APTA)-
dc.description.affiliationINIA, Estn Expt Wilson Ferreira Aldunate, Unidad Biotecnol, Rincon Del Colorado, Uruguay-
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Tecnol, Lab Bioquim Microrganismos & Plantas, Jaboticabal, Brazil-
dc.description.affiliationEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Mandioca & Fruticultura, Nucl Tecnol Recursos Gen & Desenvolvimento Varien, Cruz Das Almas, BA, Brazil-
dc.description.affiliationEstacao Expt Citricultura, São Paulo, Brazil-
dc.description.affiliationUniv São Paulo, Escola Super Agr Luiz de Queiroz, São Paulo, Brazil-
dc.description.affiliationUniv Fed Vales Jequitinhonha & Mucuri, Fac Ciencias Vet, Dept Engn Florestal, Diamantina, MG, Brazil-
dc.description.affiliationCtr Citricultura Sylvio Moreira APTA, Cordeiropolis, SP, Brazil-
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Biol Aplicada Agropecuaria, Jaboticabal, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Tecnol, Lab Bioquim Microrganismos & Plantas, Jaboticabal, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Biol Aplicada Agropecuaria, Jaboticabal, Brazil-
dc.rights.accessRightsAcesso aberto-
dc.relation.ispartofElectronic Journal of Biotechnology-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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