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dc.contributor.authorAlvaredo Paixao, Douglas Antonio-
dc.contributor.authorDimitrov, Mauricio Rocha-
dc.contributor.authorPereira, Rodrigo Matheus-
dc.contributor.authorAccorsini, Fabio Raphael-
dc.contributor.authorVidotti, Maria Benincasa-
dc.contributor.authorde Macedo Lemos, Eliana Gertrudes-
dc.identifier.citationRevista Brasileira de Ciência do Solo. Vicosa: Soc Brasileira de Ciência do Solo, v. 34, n. 3, p. 773-781, 2010.-
dc.description.abstractDiesel oil is a compound derived from petroleum, consisting primarily of hydrocarbons. Poor conditions in transportation and storage of this product can contribute significantly to accidental spills causing serious ecological problems in soil and water and affecting the diversity of the microbial environment. The cloning and sequencing of the 16S rRNA gene is one of the molecular techniques that allows estimation and comparison of the microbial diversity in different environmental samples. The aim of this work was to estimate the diversity of microorganisms from the Bacteria domain in a consortium specialized in diesel oil degradation through partial sequencing of the 16S rRNA gene. After the extraction of DNA metagenomics, the material was amplified by PCR reaction using specific oligonucleotide primers for the 16S rRNA gene. The PCR products were cloned into a pGEM-T-Easy vector (Promega), and Escherichia coli was used as the host cell for recombinant DNAs. The partial clone sequencing was obtained using universal oligonucleotide primers from the vector. The genetic library obtained generated 431 clones. All the sequenced clones presented similarity to phylum Proteobacteria, with Gammaproteobacteria the most present group (49.8 % of the clones), followed by Alphaproteobacteira (44.8 %) and Betaproteobacteria (5.4 %). The Pseudomonas genus was the most abundant in the metagenomic library, followed by the Parvibaculum and the Sphingobium genus, respectively. After partial sequencing of the 16S rRNA, the diversity of the bacterial consortium was estimated using DOTUR software. When comparing these sequences to the database from the National Center for Biotechnology Information (NCBI), a strong correlation was found between the data generated by the software used and the data deposited in NCBI.en
dc.publisherSociedade Brasileira de Ciência do Solo-
dc.sourceWeb of Science-
dc.subject16S rRNAen
dc.titleMolecular analysis of the bacterial diversity in a specialized consortium for diesel oil degradationen
dc.title.alternativeAnálise molecular da diversidade bacteriana de um consórcio degradador de óleo dieselpt
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
dc.description.affiliationUNESP São Paulo State Univ, FCAV, Dept Biol Appl Agr & Anim Husb Dev, BR-14884900 Jaboticabal, SP, Brazil-
dc.description.affiliationUniv São Paulo, BR-05508 São Paulo, Brazil-
dc.description.affiliationEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Soybean, BR-86001970 Londrina, Parana, Brazil-
dc.description.affiliationUnespUNESP São Paulo State Univ, FCAV, Dept Biol Appl Agr & Anim Husb Dev, BR-14884900 Jaboticabal, SP, Brazil-
dc.rights.accessRightsAcesso aberto-
dc.relation.ispartofRevista Brasileira de Ciência do Solo-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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