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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/11117
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dc.contributor.authorFerrasi, Adriana Camargo-
dc.contributor.authorPinheiro, Nidia Alice-
dc.contributor.authorBarem Rabenhorst, Silvia Helena-
dc.contributor.authorCaballero, Otavia Luisa-
dc.contributor.authorRodrigues, Maria Aparecida Marchesan-
dc.contributor.authorde Carvalho, Fabricio-
dc.contributor.authorLeite, Celso Vieira de Souza-
dc.contributor.authorPitombeira Ferreira, Marcia Valeria-
dc.contributor.authorPessoa Barros, Marcos Aurelio-
dc.contributor.authorPardini, Maria Ines de Moura Campos-
dc.date.accessioned2014-05-20T13:32:36Z-
dc.date.accessioned2016-10-25T16:50:53Z-
dc.date.available2014-05-20T13:32:36Z-
dc.date.available2016-10-25T16:50:53Z-
dc.date.issued2010-01-21-
dc.identifierhttp://dx.doi.org/10.3748/wjg.v16.i3.312-
dc.identifier.citationWorld Journal of Gastroenterology. Beijing: W J G Press, v. 16, n. 3, p. 312-319, 2010.-
dc.identifier.issn1007-9327-
dc.identifier.urihttp://hdl.handle.net/11449/11117-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/11117-
dc.description.abstractAIM: To verify the methylation status of CDH1, DAPK, COX2, hMLH1 and CDKN2A genes and to evaluate their association with Helicobacter pylori (H. pylori)-cagA(+) and Epstein Barr virus (EBV) infections in gastric adenocarcinomas.METHODS: Methylation-specific PCR (MSP) assay was performed in 89 primary gastric carcinomas (intestinal and diffuse types). Microsatellite instability (MSI) analysis was performed using the BAT26 primer set and PCR products were analyzed with the ABI PRISM 3100 Genetic Analyzer using Genescan 3.7 software (Applied Biosystems). Detection of H, pylori and genotyping were performed by PCR, using specific primers for ureaseC and cagA genes. The presence of EBV was assessed by in situ hybridization. Statistical analyses were performed using the chi(2) or Fisher's exact test.RESULTS: The most frequent hypermethylated gene was COX-2 (63.5%) followed by DAPK (55.7%), CDH1 (51%), CDKN2A (36%) and hMLH1 (30.3%). Intestinal and diffuse adenocarcinomas showed different methylation profiles and there was an association between methylation of E-CDH1 and H. pylori-cagA(+) in the intestinal adenocarcinoma type. MSI was correlated with hMLH1 methylation. There was an inverse correlation between DAPK hypermethylation and MSI.CONCLUSION: We found a strong association between CDH1 methylation and H, pylori-cagA+ in intestinal-type gastric cancer, association of MSI and better prognosis and an heterogeneous COX-2 overexpression. (C) 2010 Baishideng. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.format.extent312-319-
dc.language.isoeng-
dc.publisherW J G Press-
dc.sourceWeb of Science-
dc.subjectGastric canceren
dc.subjectMethylationen
dc.subjectMicrosatellite instabilityen
dc.subjectHelicobacter pylorien
dc.subjectEpstein Barr virusen
dc.titleHelicobacter pylori and EBV in gastric carcinomas: Methylation status and microsatellite instabilityen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade Federal do Ceará (UFC)-
dc.contributor.institutionLudwig Inst Canc Res-
dc.description.affiliationUNESP São Paulo State Univ, Ctr Blood Transfus, Botucatu Med Sch, BR-18618970 Botucatu, SP, Brazil-
dc.description.affiliationUniversidade Federal do Ceará (UFC), Dept Pathol, BR-60430160 Fortaleza, Ceara, Brazil-
dc.description.affiliationUNESP São Paulo State Univ, Dept Pathol, Sch Med, BR-18618970 Botucatu, SP, Brazil-
dc.description.affiliationLudwig Inst Canc Res, New York, NY 10065 USA-
dc.description.affiliationLudwig Inst Canc Res, BR-01323903 São Paulo, Brazil-
dc.description.affiliationUNESP São Paulo State Univ, Dept Surg Gastroenterol, Sch Med, BR-18618970 Botucatu, SP, Brazil-
dc.description.affiliationUnespUNESP São Paulo State Univ, Ctr Blood Transfus, Botucatu Med Sch, BR-18618970 Botucatu, SP, Brazil-
dc.description.affiliationUnespUNESP São Paulo State Univ, Dept Pathol, Sch Med, BR-18618970 Botucatu, SP, Brazil-
dc.description.affiliationUnespUNESP São Paulo State Univ, Dept Surg Gastroenterol, Sch Med, BR-18618970 Botucatu, SP, Brazil-
dc.identifier.doi10.3748/wjg.v16.i3.312-
dc.identifier.wosWOS:000273884700006-
dc.rights.accessRightsAcesso aberto-
dc.relation.ispartofWorld Journal of Gastroenterology-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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