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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/111239
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dc.contributor.authorGreenwood, Mingkwan-
dc.contributor.authorBordieri, Loredana-
dc.contributor.authorGreenwood, Michael P.-
dc.contributor.authorMelo, Mariana Rosso-
dc.contributor.authorColombari, Debora S. A.-
dc.contributor.authorColombari, Eduardo-
dc.contributor.authorPaton, Julian F. R.-
dc.contributor.authorMurphy, David-
dc.date.accessioned2014-12-03T13:07:05Z-
dc.date.accessioned2016-10-25T19:48:16Z-
dc.date.available2014-12-03T13:07:05Z-
dc.date.available2016-10-25T19:48:16Z-
dc.date.issued2014-03-12-
dc.identifierhttp://dx.doi.org/10.1523/JNEUROSCI.4343-13.2014-
dc.identifier.citationJournal Of Neuroscience. Washington: Soc Neuroscience, v. 34, n. 11, p. 3810-3820, 2014.-
dc.identifier.issn0270-6474-
dc.identifier.urihttp://hdl.handle.net/11449/111239-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/111239-
dc.description.abstractArginine vasopressin (AVP) is a neurohypophysial hormone regulating hydromineral homeostasis. Here we show that the mRNA encoding cAMP responsive element-binding protein-3 like-1 (CREB3L1), a transcription factor of the CREB/activating transcription factor (ATF) family, increases in expression in parallel with AVP expression in supraoptic nuclei (SONs) and paraventicular nuclei (PVNs) of dehydrated (DH) and salt-loaded (SL) rats, compared with euhydrated (EH) controls. In EH animals, CREB3L1 protein is expressed in glial cells, but only at a low level in SON and PVN neurons, whereas robust upregulation in AVP neurons accompanied DH and SL rats. Concomitantly, CREB3L1 is activated by cleavage, with the N-terminal domain translocating from the Golgi, via the cytosol, to the nucleus. We also show that CREB3L1 mRNA levels correlate with AVP transcription level in SONs and PVNs following sodium depletion, and as a consequence of diurnal rhythm in the suprachiasmatic nucleus. We tested the hypothesis that CREB3L1 activates AVP gene transcription. Both full-length and constitutively active forms of CREB3L1 (CREB3L1CA) induce the expression of rat AVP promoter-luciferase reporter constructs, whereas a dominant-negative mutant reduces expression. Rat AVP promoter deletion constructs revealed that CRE-like and G-box sequences in the region between -170 and -120 bp are important for CREB3L1 actions. Direct binding of CREB3L1 to the AVP promoter was shown by chromatin immunoprecipitation both in vitro and in the SON itself. Injection of a lentiviral vector expressing CREB3L1CA into rat SONs and PVNs resulted in increased AVP biosynthesis. We thus identify CREB3L1 as a regulator of AVP transcription in the rat hypothalamus.en
dc.description.sponsorshipMedical Research Council-
dc.description.sponsorshipBiotechnology and Biological Sciences Research Council-
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipUniversity of Malaya (HIR)-
dc.format.extent3810-3820-
dc.language.isoeng-
dc.publisherSoc Neuroscience-
dc.sourceWeb of Science-
dc.subjectCREB3L1en
dc.subjecthyperosmotic stressen
dc.subjecthypothalamusen
dc.subjecttranscriptionen
dc.subjectvasopressinen
dc.titleTranscription Factor CREB3L1 Regulates Vasopressin Gene Expression in the Rat Hypothalamusen
dc.typeoutro-
dc.contributor.institutionUniv Bristol-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniv Malaya-
dc.description.affiliationUniv Bristol, Sch Clin Sci, Bristol BS1 3NY, Avon, England-
dc.description.affiliationSao Paulo State Univ, Dept Physiol & Pathol, Sch Dent, BR-14801385 Sao Paulo, Brazil-
dc.description.affiliationUniv Bristol, Sch Physiol & Pharmacol, Bristol BS8 1TD, Avon, England-
dc.description.affiliationUniv Malaya, Dept Physiol, Kuala Lumpur 50603, Malaysia-
dc.description.affiliationUnespSao Paulo State Univ, Dept Physiol & Pathol, Sch Dent, BR-14801385 Sao Paulo, Brazil-
dc.description.sponsorshipIdMedical Research CouncilG0700954-
dc.description.sponsorshipIdBiotechnology and Biological Sciences Research CouncilBB/G006156/1-
dc.description.sponsorshipIdBiotechnology and Biological Sciences Research CouncilBB/J01515/1-
dc.description.sponsorshipIdFAPESP: 11/50770-1-
dc.description.sponsorshipIdUniversity of Malaya (HIR)H-20001-E000086-
dc.identifier.doi10.1523/JNEUROSCI.4343-13.2014-
dc.identifier.wosWOS:000332879500002-
dc.rights.accessRightsAcesso restrito-
dc.identifier.fileWOS000332879500002.pdf-
dc.relation.ispartofJournal of Neuroscience-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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