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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/111420
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dc.contributor.authorGoncalves, Luis Moreira-
dc.contributor.authorCallera, Welder F. A.-
dc.contributor.authorSotomayor, Maria Del Pilar Taboada-
dc.contributor.authorBueno, Paulo Roberto-
dc.date.accessioned2014-12-03T13:08:38Z-
dc.date.accessioned2016-10-25T20:08:28Z-
dc.date.available2014-12-03T13:08:38Z-
dc.date.available2016-10-25T20:08:28Z-
dc.date.issued2014-01-01-
dc.identifierhttp://dx.doi.org/10.1016/j.elecom.2013.11.022-
dc.identifier.citationElectrochemistry Communications. New York: Elsevier Science Inc, v. 38, p. 131-133, 2014.-
dc.identifier.issn1388-2481-
dc.identifier.urihttp://hdl.handle.net/11449/111420-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/111420-
dc.description.abstractA biosensor for penicillin G was created by immobilizing penicillinase to a gold electrode by means of a cysteine self-assembled monolayer. The biosensor amperometrically monitored the catalytic hydrolysis of penicillin in a very sensible manner. Furthermore, it was successfully used to measure the Michaelis-Menten enzymatic constant and a low limit of detection of 4.5 nM was obtained. (C) 2013 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipSao Paulo State University (UNESP)-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.description.sponsorshipPortuguese Fundacao para a Ciencia e a Tecnologia (FCT)-
dc.format.extent131-133-
dc.language.isoeng-
dc.publisherElsevier B.V.-
dc.sourceWeb of Science-
dc.subjectCysteineen
dc.subjectMetallo-beta-lactamaseen
dc.subjectSelf-assembled monolayer (SAM)en
dc.subjectbeta-Lactam antibioticen
dc.subjectLineweaver-Burk ploten
dc.titlePenicillinase-based amperometric biosensor for penicillin Gen
dc.typeoutro-
dc.contributor.institutionUniv Porto-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUniv Porto, Fac Ciencias, Dept Quim & Bioquim, REQUIMTE, P-4169007 Oporto, Portugal-
dc.description.affiliationUniv Estadual Paulista, Inst Quim, BR-14800900 Sao Paulo, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Inst Quim, BR-14800900 Sao Paulo, Brazil-
dc.description.sponsorshipIdPortuguese Fundacao para a Ciencia e a Tecnologia (FCT)SFRH/BPD/76544/2011-
dc.identifier.doi10.1016/j.elecom.2013.11.022-
dc.identifier.wosWOS:000331504300035-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofElectrochemistry Communications-
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