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DC Field | Value | Language |
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dc.contributor.author | Goncalves, Luis Moreira | - |
dc.contributor.author | Callera, Welder F. A. | - |
dc.contributor.author | Sotomayor, Maria Del Pilar Taboada | - |
dc.contributor.author | Bueno, Paulo Roberto | - |
dc.date.accessioned | 2014-12-03T13:08:38Z | - |
dc.date.accessioned | 2016-10-25T20:08:28Z | - |
dc.date.available | 2014-12-03T13:08:38Z | - |
dc.date.available | 2016-10-25T20:08:28Z | - |
dc.date.issued | 2014-01-01 | - |
dc.identifier | http://dx.doi.org/10.1016/j.elecom.2013.11.022 | - |
dc.identifier.citation | Electrochemistry Communications. New York: Elsevier Science Inc, v. 38, p. 131-133, 2014. | - |
dc.identifier.issn | 1388-2481 | - |
dc.identifier.uri | http://hdl.handle.net/11449/111420 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/111420 | - |
dc.description.abstract | A biosensor for penicillin G was created by immobilizing penicillinase to a gold electrode by means of a cysteine self-assembled monolayer. The biosensor amperometrically monitored the catalytic hydrolysis of penicillin in a very sensible manner. Furthermore, it was successfully used to measure the Michaelis-Menten enzymatic constant and a low limit of detection of 4.5 nM was obtained. (C) 2013 Elsevier B.V. All rights reserved. | en |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
dc.description.sponsorship | Sao Paulo State University (UNESP) | - |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | - |
dc.description.sponsorship | Portuguese Fundacao para a Ciencia e a Tecnologia (FCT) | - |
dc.format.extent | 131-133 | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.source | Web of Science | - |
dc.subject | Cysteine | en |
dc.subject | Metallo-beta-lactamase | en |
dc.subject | Self-assembled monolayer (SAM) | en |
dc.subject | beta-Lactam antibiotic | en |
dc.subject | Lineweaver-Burk plot | en |
dc.title | Penicillinase-based amperometric biosensor for penicillin G | en |
dc.type | outro | - |
dc.contributor.institution | Univ Porto | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.description.affiliation | Univ Porto, Fac Ciencias, Dept Quim & Bioquim, REQUIMTE, P-4169007 Oporto, Portugal | - |
dc.description.affiliation | Univ Estadual Paulista, Inst Quim, BR-14800900 Sao Paulo, Brazil | - |
dc.description.affiliationUnesp | Univ Estadual Paulista, Inst Quim, BR-14800900 Sao Paulo, Brazil | - |
dc.description.sponsorshipId | Portuguese Fundacao para a Ciencia e a Tecnologia (FCT)SFRH/BPD/76544/2011 | - |
dc.identifier.doi | 10.1016/j.elecom.2013.11.022 | - |
dc.identifier.wos | WOS:000331504300035 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Electrochemistry Communications | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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