Please use this identifier to cite or link to this item:
http://acervodigital.unesp.br/handle/11449/111790
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Silva-Frade, Camila | - |
dc.contributor.author | Gameiro, Roberto | - |
dc.contributor.author | Okamura, Lucas Hidenori | - |
dc.contributor.author | Flores, Eduardo Furtado | - |
dc.contributor.author | Cardoso, Tereza Cristina | - |
dc.date.accessioned | 2014-12-03T13:08:59Z | - |
dc.date.accessioned | 2016-10-25T20:09:44Z | - |
dc.date.available | 2014-12-03T13:08:59Z | - |
dc.date.available | 2016-10-25T20:09:44Z | - |
dc.date.issued | 2014-08-01 | - |
dc.identifier | http://dx.doi.org/10.1016/j.mcp.2013.10.005 | - |
dc.identifier.citation | Molecular And Cellular Probes. London: Academic Press Ltd- Elsevier Science Ltd, v. 28, n. 4, p. 113-117, 2014. | - |
dc.identifier.issn | 0890-8508 | - |
dc.identifier.uri | http://hdl.handle.net/11449/111790 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/111790 | - |
dc.description.abstract | In vitro-produced bovine embryos become infected after exposure to bovine Herpesvirus type 5 (BoHV-5), yet no changes in developmental rates, mitochondrial activity and inhibition of apoptosis are detected in comparison to unexposed embryos. Thus, the aim of the present study was to assess the transcription of mitochondria-mediated apoptosis genes using TaqMan real-time polymerase chain reaction. Transcripts of mcl-1, caspase-2, -3, Apaf-1 and Bax genes were measured after exposure to BoHV-5 in vitro. Mitochondrial dehydrogenase activity was evaluated by MIT test and compared between groups of exposed and unexposed embryos, at day 7 of development. The rate of oocyte maturation was assessed by the extrusion of the first polar body. In summary, BoHV-5 exposed embryos retained their viability, mitochondrial dehydrogenase activity and displayed up-regulation of transcription of survival mcl-1 gene and down-regulation of Bax transcription in relation to mitochondria-mediated pathway which might improve embryo viability. These findings demonstrate that BoHV-5 exposed embryos maintain their viability and mitochondrial dehydrogenase activity with no compromise of embryos produced in vitro. (c) 2013 Elsevier Ltd. All rights reserved. | en |
dc.format.extent | 113-117 | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.source | Web of Science | - |
dc.subject | Programmed cell death | en |
dc.subject | Apoptosis | en |
dc.subject | Alphaherpesvirus | en |
dc.subject | Bovine embryos | en |
dc.subject | Mitochondrion | en |
dc.title | Programmed cell death-associated gene transcripts in bovine embryos exposed to bovine Herpesvirus type 5 | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.description.affiliation | UNESP Sao Paulo State Univ, Coll Vet Med, Lab Anim Virol & Cell Culture, BR-16050680 Sao Paulo, Brazil | - |
dc.description.affiliation | UNESP Sao Paulo State Univ, Univ Fed Santa Maria, Dept Vet Prevent Med, Coll Vet Med,UFSM, BR-97115900 Santa Maria, RS, Brazil | - |
dc.description.affiliationUnesp | UNESP Sao Paulo State Univ, Coll Vet Med, Lab Anim Virol & Cell Culture, BR-16050680 Sao Paulo, Brazil | - |
dc.description.affiliationUnesp | UNESP Sao Paulo State Univ, Univ Fed Santa Maria, Dept Vet Prevent Med, Coll Vet Med,UFSM, BR-97115900 Santa Maria, RS, Brazil | - |
dc.identifier.doi | 10.1016/j.mcp.2013.10.005 | - |
dc.identifier.wos | WOS:000336948500002 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Molecular and Cellular Probes | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.