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dc.contributor.authorFranco Nunes, Francis Morais-
dc.contributor.authorPaulino Simoes, Zila Luz-
dc.identifier.citationInsect Biochemistry and Molecular Biology. Oxford: Pergamon-Elsevier B.V. Ltd, v. 39, n. 2, p. 157-160, 2009.-
dc.description.abstractin the Apis mellifera post-genomic era, RNAi protocols have been used in functional approaches. However, sample manipulation and invasive methods such as injection of double-stranded RNA (dsRNA) can compromise physiology and survival. To circumvent these problems, we developed a non-invasive method for honeybee gene knockdown, using a well-established vitellogenin RNAi system as a model. Second instar larvae received dsRNA for vitellogenin (dsVg-RNA) in their natural diet. For exogenous control, larvae received dsRNA for GFP (dsGFP-RNA). Untreated larvae formed another control group. Around 60% of the treated larvae naturally developed until adult emergence when 0.5 mu g of dsVg-RNA or dsGFP-RNA was offered while no larvae that received 3.0 mu g of dsRNA reached pupal stages. Diet dilution did not affect the removal rates. Viability depends not only on the delivered doses but also on the internal conditions of colonies. The weight of treated and untreated groups showed no statistical differences. This showed that RNAi ingestion did not elicit drastic collateral effects. Approximately 90% of vitellogenin transcripts from 7-day-old workers were silenced compared to controls. A large number of samples are handled in a relatively short time and smaller quantities of RNAi molecules are used compared to invasive methods. These advantages culminate in a versatile and a cost-effective approach. (c) 2008 Elsevier Ltd. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.publisherPergamon-Elsevier B.V. Ltd-
dc.sourceWeb of Science-
dc.subjectApis melliferaen
dc.subjectHoney beeen
dc.subjectRNAi feedingen
dc.subjectGene knockdownen
dc.subjectNon-invasive methoden
dc.subjectOff-target effectsen
dc.subjectEnvironmental RNAien
dc.titleA non-invasive method for silencing gene transcription in honeybees maintained under natural conditionsen
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Biol Aplicada & Agropecuaria, Jaboticabal, SP, Brazil-
dc.description.affiliationUniv São Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP, Brazil-
dc.description.affiliationUniv São Paulo, Fac Filosofia Ciencias & Letras Ribeirao Pret, Dept Biol, BR-14049 Ribeirao Preto, SP, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Biol Aplicada & Agropecuaria, Jaboticabal, SP, Brazil-
dc.description.sponsorshipIdFAPESP: 05/03926-5-
dc.description.sponsorshipIdCNPq: 142034/2003-7-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofInsect Biochemistry and Molecular Biology-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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