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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/112405
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dc.contributor.authorColenci, Renato-
dc.contributor.authorSilva Assuncao, Luciana Reichert da-
dc.contributor.authorMogami Bomfim, Suely Regina-
dc.contributor.authorGolim, Marjorie de Assis-
dc.contributor.authorDeffune, Elenice-
dc.contributor.authorPenha Oliveira, Sandra Helena-
dc.date.accessioned2014-12-03T13:10:41Z-
dc.date.accessioned2016-10-25T20:11:07Z-
dc.date.available2014-12-03T13:10:41Z-
dc.date.available2016-10-25T20:11:07Z-
dc.date.issued2014-03-01-
dc.identifierhttp://dx.doi.org/10.1016/j.archoralbio.2013.11.017-
dc.identifier.citationArchives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 59, n. 3, p. 268-276, 2014.-
dc.identifier.issn0003-9969-
dc.identifier.urihttp://hdl.handle.net/11449/112405-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/112405-
dc.description.abstractObjective: The aim of this study was to evaluate, in vitro, the role of bFGF in the proliferation and expression of collagen type I and fibronectin of dog bone marrow mesenchymal stem cells (dBMMSCs) in comparison with the expression of the same proteins in dog periodontal fibroblasts (dPLFs).Design: dBMMSCs from the iliac crest were cultivated in Dulbecco's Modified Eagle's Medium (DMEM). Flow cytometry analysis (FCA) was used to characterize dBMMSC. Cells were stimulated with bFGF (1, 5 and 10 ng/mL) after 24 and 48 h. Real time RT-PCR was performed to verify collagen type I and fibronectin expressions. MTT assay was used to confirm cellular proliferation. Statistical analyses were performed (ANOVA and Kruskal Wallis tests; p < 0.05).Results: FCA showed 55.98% of CD34+ and 32.67% of CD90+ after bone marrow aspiration; 3.33% of CD34+ and 33.0% of CD90+ before P1. After P2, 10.54% of dBMMSCs expressed CD90, whereas after P3, this number decreased to 1.58%. dPLFs presented 4.04% of CD90+ and 1.05% of CD34+ after P3. MU evaluation showed increase in dBMSC proliferation with 5 ng/mL bFGF-stimulus after 24-h. Both collagen land fibronectin expression were very similar between the two cells groups after 24-h stimulation with 1 ng/mL bFGF concentration. Fibronectin and collagen I expressions were higher after 24-h stimulation with 5 ng/mL bFGF.Conclusion: dBMMSCs (1 ng/mL-bFGF stimulus after 24 h) are very similar to dPLFs as regards morphological and immunostaining characteristics, and collagen and/or fibronectin production. The dBMMSCs presented the highest protein expression rates with 5 ng/mL-bFGF stimulus after 24-h. (C) 2013 Elsevier Ltd. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
dc.format.extent268-276-
dc.language.isoeng-
dc.publisherElsevier B.V.-
dc.sourceWeb of Science-
dc.subjectBone marrowen
dc.subjectMesenchymal stem cellsen
dc.subjectPeriodontal ligamenten
dc.subjectFibroblastsen
dc.subjectbFGFen
dc.titleBone marrow mesenchymal stem cells stimulated by bFGF up-regulated protein expression in comparison with periodontal fibroblasts in vitroen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade Federal do Paraná (UFPR)-
dc.description.affiliationUNESP Univ Estadual Paulista, DDS, Sch Dent, Sao Paulo, Brazil-
dc.description.affiliationUniv Fed Parana, UFPR, Sch Dent, Dept Stornatol, BR-80060000 Curitiba, Parana, Brazil-
dc.description.affiliationUNESP Univ Estadual Paulista, Sch Vet Med, Dept Clin Surg & Anim Reprod, Sao Paulo, Brazil-
dc.description.affiliationUNESP Univ Estadual Paulista, Sch Med, Botucatu Blood Ctr, Lab Flow Cytometry, Sao Paulo, Brazil-
dc.description.affiliationUNESP Univ Estadual Paulista, Sch Med, Botucatu Blood Ctr, Lab Cellular Engn, Sao Paulo, Brazil-
dc.description.affiliationUNESP Univ Estadual Paulista, Dept Basic Sci, Sch Dent, Sao Paulo, Brazil-
dc.description.affiliationUnespUNESP Univ Estadual Paulista, DDS, Sch Dent, Sao Paulo, Brazil-
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Sch Vet Med, Dept Clin Surg & Anim Reprod, Sao Paulo, Brazil-
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Sch Med, Botucatu Blood Ctr, Lab Flow Cytometry, Sao Paulo, Brazil-
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Sch Med, Botucatu Blood Ctr, Lab Cellular Engn, Sao Paulo, Brazil-
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Dept Basic Sci, Sch Dent, Sao Paulo, Brazil-
dc.description.sponsorshipIdFAPESP: 06/59420-5-
dc.identifier.doi10.1016/j.archoralbio.2013.11.017-
dc.identifier.wosWOS:000333489600005-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofArchives of Oral Biology-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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