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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/112406
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dc.contributor.authorMelo, L. M.-
dc.contributor.authorPerosso, J.-
dc.contributor.authorAlmeida, B. F. M.-
dc.contributor.authorSilva, K. L. O.-
dc.contributor.authorSomenzari, M. A.-
dc.contributor.authorLima, Valéria Marçal Felix de-
dc.date.accessioned2014-12-03T13:10:41Z-
dc.date.accessioned2016-10-25T20:11:07Z-
dc.date.available2014-12-03T13:10:41Z-
dc.date.available2016-10-25T20:11:07Z-
dc.date.issued2014-02-01-
dc.identifierhttp://dx.doi.org/10.1016/j.intimp.2013.12.012-
dc.identifier.citationInternational Immunopharmacology. Amsterdam: Elsevier Science Bv, v. 18, n. 2, p. 373-378, 2014.-
dc.identifier.issn1567-5769-
dc.identifier.urihttp://hdl.handle.net/11449/112406-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/112406-
dc.description.abstractLeishmania (L.) chagasi is the etiologic agent of visceral leishmaniasis (VL) that can be transmitted to humans and dogs. VL in Brazil represents a serious public health problem; therefore, it is important to study new alternatives to treat infected dogs. In dogs, the therapeutic arsenal against canine VL is limited. The immunomodulator protein aggregate magnesium-ammonium phospholinoleate-palmitoleate anhydride (P-MAPA) improves immunocompetence when the immune system is impaired, but its dependence on Toll-like receptors (TLRs) and the mechanisms involved in immune response remain unclear. The in vitro action of P-MAPA on the expression of TLR2 and TLR4, reactive oxygen species (ROS), nitric oxide (NO) and p38 mitogen-activated protein kinase (p38 MAPK) and IKK phosphorylation was studied in mononuclear cells from peripheral blood and macrophages from healthy and Leishmania-infected dogs. The PBMC or macrophages were isolated and cultured with different concentrations of P-MAPA (20,100 and 200 mu g/ml) in a humid environment at 37 degrees C with 5% CO2. Observation revealed that Leishmania-infected dogs showed a decrease in TLR2 in macrophages compared with healthy dogs and in induction with P-MAPA. ROS were increased in PBMCs from Leishmania spp.-infected dogs compared with healthy dogs and P-MAPA improved ROS production. NO production was increased in culture supernatant from macrophages stimulated by P-MAPA in both healthy and Leishmania spp. infected dogs. Treatment of macrophages from healthy dogs with immunomodulatory P-MAPA induced p38 MAPK and IKK phosphorylation, suggesting signal transduction by this pathway. These findings suggest that P-MAPA has potential as a therapeutic drug in the treatment of canine visceral leishmaniasis. (C) 2014 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.format.extent373-378-
dc.language.isoeng-
dc.publisherElsevier B.V.-
dc.sourceWeb of Science-
dc.subjectLeishmania sp.en
dc.subjectP-MAPAen
dc.subjectDogen
dc.subjectImmunomodulatoryen
dc.subjectTLRen
dc.titleEffects of P-MAPA immunomodulator on Toll-like receptor 2, ROS, nitric oxide, MAPKp38 and IKK in PBMC and macrophages from dogs with visceral leishmaniasisen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUniv Estadual Paulista, Fac Med Vet, Posgrad Ciencia Anim, Aracatuba, SP, Brazil-
dc.description.affiliationUniv Estadual Paulista, Fac Med Vet, Dept Clin Cirugia & Reprod Anim, Aracatuba, SP, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Fac Med Vet, Posgrad Ciencia Anim, Aracatuba, SP, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Fac Med Vet, Dept Clin Cirugia & Reprod Anim, Aracatuba, SP, Brazil-
dc.description.sponsorshipIdFAPESP: 09/50426-9-
dc.description.sponsorshipIdFAPESP: 10/13166-6-
dc.identifier.doi10.1016/j.intimp.2013.12.012-
dc.identifier.wosWOS:000331412900023-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofInternational Immunopharmacology-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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