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http://acervodigital.unesp.br/handle/11449/112406
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DC Field | Value | Language |
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dc.contributor.author | Melo, L. M. | - |
dc.contributor.author | Perosso, J. | - |
dc.contributor.author | Almeida, B. F. M. | - |
dc.contributor.author | Silva, K. L. O. | - |
dc.contributor.author | Somenzari, M. A. | - |
dc.contributor.author | Lima, Valéria Marçal Felix de | - |
dc.date.accessioned | 2014-12-03T13:10:41Z | - |
dc.date.accessioned | 2016-10-25T20:11:07Z | - |
dc.date.available | 2014-12-03T13:10:41Z | - |
dc.date.available | 2016-10-25T20:11:07Z | - |
dc.date.issued | 2014-02-01 | - |
dc.identifier | http://dx.doi.org/10.1016/j.intimp.2013.12.012 | - |
dc.identifier.citation | International Immunopharmacology. Amsterdam: Elsevier Science Bv, v. 18, n. 2, p. 373-378, 2014. | - |
dc.identifier.issn | 1567-5769 | - |
dc.identifier.uri | http://hdl.handle.net/11449/112406 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/112406 | - |
dc.description.abstract | Leishmania (L.) chagasi is the etiologic agent of visceral leishmaniasis (VL) that can be transmitted to humans and dogs. VL in Brazil represents a serious public health problem; therefore, it is important to study new alternatives to treat infected dogs. In dogs, the therapeutic arsenal against canine VL is limited. The immunomodulator protein aggregate magnesium-ammonium phospholinoleate-palmitoleate anhydride (P-MAPA) improves immunocompetence when the immune system is impaired, but its dependence on Toll-like receptors (TLRs) and the mechanisms involved in immune response remain unclear. The in vitro action of P-MAPA on the expression of TLR2 and TLR4, reactive oxygen species (ROS), nitric oxide (NO) and p38 mitogen-activated protein kinase (p38 MAPK) and IKK phosphorylation was studied in mononuclear cells from peripheral blood and macrophages from healthy and Leishmania-infected dogs. The PBMC or macrophages were isolated and cultured with different concentrations of P-MAPA (20,100 and 200 mu g/ml) in a humid environment at 37 degrees C with 5% CO2. Observation revealed that Leishmania-infected dogs showed a decrease in TLR2 in macrophages compared with healthy dogs and in induction with P-MAPA. ROS were increased in PBMCs from Leishmania spp.-infected dogs compared with healthy dogs and P-MAPA improved ROS production. NO production was increased in culture supernatant from macrophages stimulated by P-MAPA in both healthy and Leishmania spp. infected dogs. Treatment of macrophages from healthy dogs with immunomodulatory P-MAPA induced p38 MAPK and IKK phosphorylation, suggesting signal transduction by this pathway. These findings suggest that P-MAPA has potential as a therapeutic drug in the treatment of canine visceral leishmaniasis. (C) 2014 Elsevier B.V. All rights reserved. | en |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
dc.format.extent | 373-378 | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.source | Web of Science | - |
dc.subject | Leishmania sp. | en |
dc.subject | P-MAPA | en |
dc.subject | Dog | en |
dc.subject | Immunomodulatory | en |
dc.subject | TLR | en |
dc.title | Effects of P-MAPA immunomodulator on Toll-like receptor 2, ROS, nitric oxide, MAPKp38 and IKK in PBMC and macrophages from dogs with visceral leishmaniasis | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.description.affiliation | Univ Estadual Paulista, Fac Med Vet, Posgrad Ciencia Anim, Aracatuba, SP, Brazil | - |
dc.description.affiliation | Univ Estadual Paulista, Fac Med Vet, Dept Clin Cirugia & Reprod Anim, Aracatuba, SP, Brazil | - |
dc.description.affiliationUnesp | Univ Estadual Paulista, Fac Med Vet, Posgrad Ciencia Anim, Aracatuba, SP, Brazil | - |
dc.description.affiliationUnesp | Univ Estadual Paulista, Fac Med Vet, Dept Clin Cirugia & Reprod Anim, Aracatuba, SP, Brazil | - |
dc.description.sponsorshipId | FAPESP: 09/50426-9 | - |
dc.description.sponsorshipId | FAPESP: 10/13166-6 | - |
dc.identifier.doi | 10.1016/j.intimp.2013.12.012 | - |
dc.identifier.wos | WOS:000331412900023 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | International Immunopharmacology | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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