Please use this identifier to cite or link to this item:
http://acervodigital.unesp.br/handle/11449/112504
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Nogueira, Andressa Vilas Boas | - |
dc.contributor.author | Nokhbehsaim, Marjan | - |
dc.contributor.author | Eick, Sigrun | - |
dc.contributor.author | Bourauel, Christoph | - |
dc.contributor.author | Jaeger, Andreas | - |
dc.contributor.author | Jepsen, Soren | - |
dc.contributor.author | Rossa, Carlos | - |
dc.contributor.author | Deschner, James | - |
dc.contributor.author | Cirelli, Joni Augusto | - |
dc.date.accessioned | 2014-12-03T13:10:46Z | - |
dc.date.accessioned | 2016-10-25T20:11:21Z | - |
dc.date.available | 2014-12-03T13:10:46Z | - |
dc.date.available | 2016-10-25T20:11:21Z | - |
dc.date.issued | 2014-01-01 | - |
dc.identifier | http://dx.doi.org/10.1155/2014/425421 | - |
dc.identifier.citation | Mediators Of Inflammation. New York: Hindawi Publishing Corporation, 10 p., 2014. | - |
dc.identifier.issn | 0962-9351 | - |
dc.identifier.uri | http://hdl.handle.net/11449/112504 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/112504 | - |
dc.description.abstract | The present study aimed to evaluate in vitro whether biomechanical loading modulates proinflammatory and bone remodeling mediators production by periodontal ligament (PDL) cells in the presence of bacterial challenge. Cells were seeded on BioFlex culture plates and exposed to Fusobacterium nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL) and high (CTSH) magnitudes for 1 and 3 days. Synthesis of cyclooxygenase-2 (COX2) and prostaglandin E2 (PGE2) was evaluated by ELISA. Gene expression and protein secretion of osteoprotegerin (OPG) and receptor activator of nuclear factor kappa-B ligand (RANKL) were evaluated by quantitative RT-PCR and ELISA, respectively. F. nucleatum increased the production of COX2 and PGE2, which was further increased by CTS. F. nucleatum-induced increase of PGE2 synthesis was significantly (P < 0.05) increased when CTSH was applied at 1 and 3 days. In addition, CTSH inhibited the F. nucleatum-induced upregulation of OPG at 1 and 3 days, thereby increasing the RANKL/OPG ratio. OPG and RANKL mRNA results correlated with the protein results. In summary, our findings provide original evidence that CTS can enhance bacterial-induced syntheses of molecules associated with inflammation and bone resorption by PDL cells. Therefore, biomechanical, such as orthodontic or occlusal, loading may enhance the bacterial-induced inflammation and destruction in periodontitis. | en |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | - |
dc.description.sponsorship | German Research Foundation | - |
dc.description.sponsorship | Medical Faculty of the University of Bonn | - |
dc.format.extent | 10 | - |
dc.language.iso | eng | - |
dc.publisher | Hindawi Publishing Corporation | - |
dc.source | Web of Science | - |
dc.title | Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | Univ Bonn | - |
dc.contributor.institution | Univ Bern | - |
dc.description.affiliation | Univ Estadual Paulista, UNESP, Sch Dent Araraquara, Dept Diag & Surg, BR-14801903 Araraquara, SP, Brazil | - |
dc.description.affiliation | Univ Bonn, Ctr Dentomaxillofacial Med, Sect Expt Dentomaxillofacial Med, D-53111 Bonn, Germany | - |
dc.description.affiliation | Univ Bonn, Ctr Dentomaxillofacial Med, Clin Res Unit 208, D-53111 Bonn, Germany | - |
dc.description.affiliation | Univ Bern, Dept Periodontol, CH-3010 Bern, Switzerland | - |
dc.description.affiliation | Univ Bonn, Ctr Dentomaxillofacial Med, D-53111 Bonn, Germany | - |
dc.description.affiliation | Univ Bonn, Ctr Dentomaxillofacial Med, Dept Orthodont, D-53111 Bonn, Germany | - |
dc.description.affiliation | Univ Bonn, Ctr Dentomaxillofacial Med, Dept Periodontol Operat & Prevent Dent, D-53111 Bonn, Germany | - |
dc.description.affiliationUnesp | Univ Estadual Paulista, UNESP, Sch Dent Araraquara, Dept Diag & Surg, BR-14801903 Araraquara, SP, Brazil | - |
dc.description.sponsorshipId | FAPESP: 10/07771-4 | - |
dc.description.sponsorshipId | FAPESP: 11/13752-5 | - |
dc.description.sponsorshipId | CAPES: CAPES: 2385-11-2 | - |
dc.description.sponsorshipId | German Research FoundationDFG: KFO208/TP4 | - |
dc.identifier.doi | 10.1155/2014/425421 | - |
dc.identifier.wos | WOS:000337444600001 | - |
dc.rights.accessRights | Acesso aberto | - |
dc.identifier.file | WOS000337444600001.pdf | - |
dc.relation.ispartof | Mediators of Inflammation | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.