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dc.contributor.authorFernandes, Carlos A. H.-
dc.contributor.authorComparetti, Edson J.-
dc.contributor.authorBorges, Rafael J.-
dc.contributor.authorHuancahuire-Vega, Salomon-
dc.contributor.authorPonce-Soto, Luis Alberto-
dc.contributor.authorMarangoni, Sergio-
dc.contributor.authorSoares, Andreimar M.-
dc.contributor.authorFontes, Marcos R. M.-
dc.identifier.citationBiochimica Et Biophysica Acta-proteins And Proteomics. Amsterdam: Elsevier Science Bv, v. 1834, n. 12, p. 2772-2781, 2013.-
dc.description.abstractBothrops brazili is a snake found in the forests of the Amazonian region whose commercial therapeutic antibothropic serum has low efficacy for local myotoxic effects, resulting in an important public health problem in this area. Catalytically inactive phospholipases A(2)-like (Lys49-PLA(2)s) are among the main components from Bothrops genus venoms and are capable of causing drastic myonecrosis. Several studies have shown that the C-terminal region of these toxins, which includes a variable combination of positively charged and hydrophobic residues, is responsible for their activity. In this work we describe the crystal structures of two Lys49-PLA(2)s (BbTX-II and MIX-II) from B. brazili venom and a comprehensive structural comparison with several Lys49-PLA(2)s. Based on these results, two independent sites of interaction were identified between protein and membrane which leads to the proposition of a new myotoxic mechanism for bothropic Lys49-PLA(2)s composed of five different steps. This proposition is able to fully explain the action of these toxins and may be useful to develop efficient inhibitors to complement the conventional antivenom administration. (C) 2013 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
dc.publisherElsevier B.V.-
dc.sourceWeb of Science-
dc.subjectPhospholipase A(2)-likeen
dc.subjectMyotoxic mechanismen
dc.subjectSnake venomen
dc.subjectX-ray crystallographyen
dc.subjectLys49-phospholipase A(2)en
dc.subjectAmazonian snakeen
dc.titleStructural bases for a complete myotoxic mechanism: Crystal structures of two non-catalytic phospholipases A(2)-like from Bothrops brazili venomen
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)-
dc.contributor.institutionUniversidade Federal de Rondônia (UNIR)-
dc.description.affiliationUniv Estadual Paulista, UNESP, Inst Biociencias, Dep Fis & Biofis, Botucatu, SP, Brazil-
dc.description.affiliationUniv Estadual Campinas, Inst Biol, Dep Bioquim, Campinas, SP, Brazil-
dc.description.affiliationUniv Estadual Campinas, Fac Ciencias Med, Dep Farmacol, Campinas, SP, Brazil-
dc.description.affiliationUniv Fed Rondonia, FIOCRUZ Rondania, Fundacao Oswaldo Cruz, Porto Velho, RO, Brazil-
dc.description.affiliationUniv Fed Rondonia, Ctr Estudos Biomol Aplicadas, Porto Velho, RO, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Inst Biociencias, Dep Fis & Biofis, Botucatu, SP, Brazil-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofBiochimica et Biophysica Acta: Proteins and Proteomics-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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