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dc.contributor.authorTakeda, Agnes A. S.-
dc.contributor.authorFreitas, Fernanda Zanolli-
dc.contributor.authorMagro, Angelo J.-
dc.contributor.authorBernardes, Natalia E.-
dc.contributor.authorFernandes, Carlos A. H.-
dc.contributor.authorGoncalves, Rodrigo D.-
dc.contributor.authorBertolini, Maria Celia-
dc.contributor.authorFontes, Marcos R. M.-
dc.identifier.citationProtein and Peptide Letters. Sharjah: Bentham Science Publ Ltd, v. 20, n. 1, p. 8-16, 2013.-
dc.description.abstractNeurospora crassa has been widely used as a model organism and contributed to the development of biochemistry and molecular biology by allowing the identification of many metabolic pathways and mechanisms responsible for gene regulation. Nuclear proteins are synthesized in the cytoplasm and need to be translocated to the nucleus to exert their functions which the importin alpha-receptor has a key role for the classical nuclear import pathway. In an attempt to get structural information of the nuclear transport process in N. crassa, we present herein the cloning, expression, purification and structural studies with N-terminally truncated IMP alpha from N. crassa (IMP alpha-Nc). Circular dichroism analysis revealed that the IMP alpha-Nc obtained is correctly folded and presents a high structural conservation compared to other importins-alpha. Dynamic light scattering, analytical size-exclusion chromatography experiments and molecular dynamics simulations indicated that the IMP alpha-Nc unbound to any ligand may present low stability in solution. The IMP alpha-Nc theoretical model displayed high similarity of its inner concave surface, which binds the cargo proteins containing the nuclear localization sequences, among IMP alpha from different species. However, the presence of non-conserved amino acids relatively close to the NLS binding region may influence the binding specificity of IMP alpha-Nc to cargo proteins.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
dc.publisherBentham Science Publ Ltd-
dc.sourceWeb of Science-
dc.subjectBiophysical characterizationen
dc.subjectclassical nuclear import pathwayen
dc.subjectheterologous expressionen
dc.subjecthomology modelingen
dc.subjectNeurospora crassaen
dc.titleBiophysical Characterization of the Recombinant Importin-alpha from Neurospora crassaen
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Dept Fis & Biofis, BR-18618970 Botucatu, SP, Brazil-
dc.description.affiliationUniv Estadual Paulista, Inst Quim, Dept Bioquim & Tecnol Quim, Araraquara, SP, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Dept Fis & Biofis, BR-18618970 Botucatu, SP, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Inst Quim, Dept Bioquim & Tecnol Quim, Araraquara, SP, Brazil-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofProtein and Peptide Letters-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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