You are in the accessibility menu

Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorGomes, M. L.-
dc.contributor.authorHatanaka, T.-
dc.contributor.authorCampos, W. N. de-
dc.contributor.authorWasko, A. P.-
dc.identifier.citationBrazilian Journal of Poultry Science. Campinas: Facta-fundacio Arnco Ciencia Tecnologia Avicolas, v. 15, n. 4, p. 329-338, 2013.-
dc.description.abstractMicrosatellite markers were analyzed in Japanese quails, Coturnix japonica, using different methodologies (PAGE and automated genotyping), in order to evaluate their use in paternity testing. Ten animal triplets composed by a female and two males were used to mate and generate an offspring. Paternity was determined in five-day-old embryos, and the data generated by fluorescent labeled and tailored primers in PCR and further automated genotyping were robust. Three microsatellite markers were polymorphic (Na = 5-8, H-E = 0.75) and no loci were found to deviate significantly from Hardy-Weinberg equilibrium or showed any evidence of linkage disequilibrium (p > 0.05). A slight heterozygote deficiency and some incompatibilities between the female known parent and its offspring that involved homozygous genotypes were observed at GUJ0001 locus and may indicate the presence of null alleles. Although a reduced set of microsatellite primers were applied, it was possible to determine the paternity of 96.87% of the embryos, using combined data of three loci. The approach was useful for parentage inferring in a captive population of C. japonica and the results evidenced a potential polyandric mating system in the species, in which no advantage mechanism of last-male sperm precedence seems to occur.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.publisherFacta-fundacio Arnco Ciência Tecnologia Avicolas-
dc.sourceWeb of Science-
dc.subjectCoturnix japonicaen
dc.subjectparentage testingen
dc.titleAssessing Paternity in Japanese Quails (Coturnix Japonica) Using Microsatellite Markers - Inferences for Its Mating System and Reproductive Successen
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)-
dc.description.affiliationUniv Estadual Paulista UNESP, Dept Genet, Inst Biociencias, BR-18618970 Botucatu, SP, Brazil-
dc.description.affiliationFed Univ Sao Carlos UFSCar, Dept Genet & Evolut, Sao Carlos, SP, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Dept Genet, Inst Biociencias, BR-18618970 Botucatu, SP, Brazil-
dc.rights.accessRightsAcesso aberto-
dc.relation.ispartofBrazilian Journal of Poultry Science-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.